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Literature summary for 2.7.1.78 extracted from

  • Martins, A.; Shuman, S.
    Characterization of a baculovirus enzyme with RNA ligase, polynucleotide 5'-Kinase, and polynucleotide 3'-phosphatase activities (2004), J. Biol. Chem., 279, 18220-18231.
    View publication on PubMed
Search for more literature for 2.7.1.78

Protein Variants

Protein Variants Comment Organism
additional information expression of wild-type C-terminal kinase domain, amino acids 394-694 with His10-tag gives a 38 kDa protein with enzymic activity. Mutant K407A of the domain has no enzymic activity. Mutation D560A has no effect unidentified baculovirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 ATP pH 5.0, 30°C unidentified baculovirus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1.25-5 mM unidentified baculovirus
Mn2+ 1.25-5 mM unidentified baculovirus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 x * 80000, recombinant protein with His10-tag, SDS-PAGE unidentified baculovirus

Organism

Organism UniProt Comment Textmining
unidentified baculovirus
-
 trifunctional enzyme with RNA ligase, polynucleotide 5'-kinase, and polynucleotide 3'-phosphatase activities
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-dephospho-DNA
-
 unidentified baculovirus ADP + 5'-phospho-DNA
-
 ?
ATP + 5'-dephospho-RNA
-
 unidentified baculovirus ADP + 5'-phospho-RNA
-
 ?
GTP + 5'-dephospho-DNA
-
 unidentified baculovirus GDP + 5'-phospho-DNA
-
 ?

Subunits

Subunits Comment Organism
? x * 80000, recombinant protein with His10-tag, SDS-PAGE unidentified baculovirus
More three enzymic activities are organized on the polypeptide in modular fashion with an autonomous N-terminal RNA-specific ligase domain, and a C-terminal kinase-phosphatase domain unidentified baculovirus

Synonyms

Synonyms Comment Organism
Rnl1
-
 unidentified baculovirus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.023
-
 ATP pH 5.0, 30°C unidentified baculovirus

pH Range

pH Minimum pH Maximum Comment Organism
4
-
 50% of maximum activity unidentified baculovirus
4.5 7
-
 unidentified baculovirus