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Literature summary for 2.7.1.78 extracted from

  • Wang, L.K.; Shuman, S.
    Mutational analysis defines the 5'-kinase and 3'-phosphatase active sites of T4 polynucleotide kinase (2002), Nucleic Acids Res., 30, 1073-1080.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
D165A site-directed mutagenesis, increased activity compared to the wild-type, no 3'-phosphatase activity remaining Tequatrovirus T4
D180A site-directed mutagenesis, slightly higher activity than the wild-type, reduced 3'-phosphatase activity Tequatrovirus T4
D250A site-directed mutagenesis, increased activity compared to the wild-type , reduced 3'-phosphatase activity Tequatrovirus T4
D254A site-directed mutagenesis, increased activity compared to the wild-type, nearly no 3'-phosphatase activity remaining Tequatrovirus T4
D278A site-directed mutagenesis, increased activity compared to the wild-type, no 3'-phosphatase activity remaining Tequatrovirus T4
K125A site-directed mutagenesis, slightly higher activity than the wild-type Tequatrovirus T4
R122A site-directed mutagenesis, slightly higher activity than the wild-type, reduced 3'-phosphatase activity Tequatrovirus T4
R126A site-directed mutagenesis, highly reduced activity Tequatrovirus T4
R176A site-directed mutagenesis, increased activity compared to the wild-type, highly reduced 3'-phosphatase activity Tequatrovirus T4
R213A site-directed mutagenesis, increased activity compared to the wild-type, no 3'-phosphatase activity remaining Tequatrovirus T4
R246A site-directed mutagenesis, increased activity compared to the wild-type, highly reduced 3'-phosphatase activity Tequatrovirus T4
R279A site-directed mutagenesis, increased activity compared to the wild-type, highly reduced 3'-phosphatase activity Tequatrovirus T4
R36A site-directed mutagenesis, similar activity than the wild-type Tequatrovirus T4
R38A site-directed mutagenesis, highly reduced activity, reduced 3'-phosphatase activity Tequatrovirus T4
S84A site-directed mutagenesis, slightly lower activity than the wild-type, reduced 3'-phosphatase activity Tequatrovirus T4

Inhibitors

Inhibitors Comment Organism Structure
diphosphate
-
Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5'-dephospho-RNA Tequatrovirus T4 involved in repair of broken RNA termini ADP + 5'-phospho-RNA
-
?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
from infected Escherichia coli
-

Reaction

Reaction Comment Organism Reaction ID
ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA 5 amino acids, Lys15, Ser116, Asp35, Arg38, and Arg126, comprise the 5'-kinase active site, structure-activity relationships Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-dephospho-RNA
-
Tequatrovirus T4 ADP + 5'-phospho-RNA
-
?
ATP + 5'-dephospho-RNA involved in repair of broken RNA termini Tequatrovirus T4 ADP + 5'-phospho-RNA
-
?
ATP + synthetic oligonucleotide
-
Tequatrovirus T4 ADP + oligonucleotide 5'-phosphate
-
?
additional information enzyme also has DNA 3'-phosphatase activity Tequatrovirus T4 ?
-
?

Synonyms

Synonyms Comment Organism
More enzyme also possesses a 3'-phosphatase activity Tequatrovirus T4