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Literature summary for 2.7.1.78 extracted from

  • Prinos, P.; Slack, C.; Lasko, D.D.
    5' Phosphorylation of DNA in mammalian cells: identification of a polymin P-precipitable polynucleotide kinase (1995), J. Cell. Biochem., 58, 115-131.
    View publication on PubMed
Search for more literature for 2.7.1.78

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 54% remaining activity without DTT Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
ammonium sulfate 50% inhibition at 8.5 mM Bos taurus
diphosphate 50% inhibition at 2.2 mM Bos taurus
EDTA complete inhibition at 25 mM Bos taurus
KCl 50% inhibition at 71 mM Bos taurus
NaCl 50% inhibition at 67 mM Bos taurus
phosphate 50% inhibition at 11 mM Bos taurus
sulfate 50% inhibition at 5.7 mM Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 oligo (dT)25 polymin P precipitable enzyme, pH 7.5, 37°C Bos taurus
0.052
-
 ATP polymin P precipitable enzyme, pH 7.5, 37°C Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates Bos taurus
Mg2+ best at 1 mM Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 sucrose density gradient centrifugation Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-

Purification (Commentary)

Purification (Comment) Organism
polymin P precipitable enzyme, 1570fold Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0002
-
 purified, polymin P precipitable enzyme, pH 5.5, 37°C Bos taurus

Storage Stability

Storage Stability Organism
0°C, partially purified enzyme, 50 mM Tris-HCl, pH 7.5, 30 mM NaCl, 0.5 mM DTT, 0.5 mM EDTA, on ice, stable for several weeks Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-dephospho-DNA
-
 Bos taurus ADP + 5'-phospho-DNA
-
 ?
ATP + nucleoside-3'-monophosphate no activity with thymidine 3'-monophosphate Bos taurus ADP + nucleoside-3',5'-diphosphate
-
 ?
ATP + oligo (dT)25
-
 Bos taurus ?
-
 ?
ATP + synthetic oligonucleotide oligo (dT)25 Bos taurus ADP + oligonucleotide 5'-phosphate
-
 ?
additional information substrate specificity Bos taurus ?
-
 ?
additional information no activity with thymidine 3'-monophosphate Bos taurus ?
-
 ?
additional information no 3'-phosphatase activity Bos taurus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9 polymin P precipitable enzyme Bos taurus

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Bos taurus