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Literature summary for 2.7.1.78 extracted from

  • Lorsch, J.R.; Szostak, J.W.
    Kinetic and thermodynamic characterization of the reaction catalyzed by a polynucleotide kinase ribozyme (1995), Biochemistry, 34, 15315-15327.
    View publication on PubMed

Application

Application Comment Organism
synthesis construction of a catalytically useful ribozyme synthetic ribozyme

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
synthetic ribozyme

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information detailed reaction kinetics, pH- and temperature-in-/dependence synthetic ribozyme
3.1
-
[gamma-S]ATP pH-independent in the range pH 6.0-8.5, 30°C synthetic ribozyme
3.4
-
ATP pH-independent in the range pH 6.0-8.5, 30°C synthetic ribozyme

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required synthetic ribozyme
Mn2+
-
synthetic ribozyme

Organism

Organism UniProt Comment Textmining
synthetic ribozyme
-
class I polynucleotide kinase
-

Reaction

Reaction Comment Organism Reaction ID
ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA reaction mechanism synthetic ribozyme

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + synthetic oligonucleotide substrate 5'-OH-(ribonucleotide)7 synthetic ribozyme ADP + oligonucleotide 5'-phosphate
-
?
additional information substrate specificity synthetic ribozyme ?
-
?
additional information binding constants of the reaction steps synthetic ribozyme ?
-
?
[gamma-S]ATP + 5'-OH-(ribonucleotide)7 best phosphate donor synthetic ribozyme [gamma-S]ADP + 5'-phospho-(ribonucleotide)7
-
?
[gamma-S]ATP + 5'-OH-(ribonucleotide)7 very low activity with [gamma-S]GTP synthetic ribozyme [gamma-S]ADP + 5'-phospho-(ribonucleotide)7
-
?

Subunits

Subunits Comment Organism
More secondary structure of the ribozyme synthetic ribozyme

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
detailed reaction kinetics, pH- and temperature-dependence synthetic ribozyme

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00005
-
ATP pH-independent, 30°C synthetic ribozyme
0.00283
-
[gamma-S]ATP pH-independent, 30°C synthetic ribozyme

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
detailed reaction kinetics, pH- and temperature-dependence synthetic ribozyme

Cofactor

Cofactor Comment Organism Structure
4-(3-bromo-8-chloro-6,11-dihydro-5H-benzo[5,6]cyclohepta[1,2-b]pyridin-11-yl)-N1-cyclohexyl-N2-[2-(1H-imidazol-4-yl)ethyl]piperazine-1,2-dicarboxamide best phosphate-donor synthetic ribozyme
ATP
-
synthetic ribozyme

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information
-
synthetic ribozyme
3.8
-
ADP pH-independent in the range pH 6.0-8.5, 30°C synthetic ribozyme