Application | Comment | Organism |
---|---|---|
synthesis | construction of a catalytically useful ribozyme | synthetic ribozyme |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
synthetic ribozyme |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | detailed reaction kinetics, pH- and temperature-in-/dependence | synthetic ribozyme | |
3.1 | - |
[gamma-S]ATP | pH-independent in the range pH 6.0-8.5, 30°C | synthetic ribozyme | |
3.4 | - |
ATP | pH-independent in the range pH 6.0-8.5, 30°C | synthetic ribozyme |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | synthetic ribozyme | |
Mn2+ | - |
synthetic ribozyme |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
synthetic ribozyme | - |
class I polynucleotide kinase | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA | reaction mechanism | synthetic ribozyme |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + synthetic oligonucleotide | substrate 5'-OH-(ribonucleotide)7 | synthetic ribozyme | ADP + oligonucleotide 5'-phosphate | - |
? | |
additional information | substrate specificity | synthetic ribozyme | ? | - |
? | |
additional information | binding constants of the reaction steps | synthetic ribozyme | ? | - |
? | |
[gamma-S]ATP + 5'-OH-(ribonucleotide)7 | best phosphate donor | synthetic ribozyme | [gamma-S]ADP + 5'-phospho-(ribonucleotide)7 | - |
? | |
[gamma-S]ATP + 5'-OH-(ribonucleotide)7 | very low activity with [gamma-S]GTP | synthetic ribozyme | [gamma-S]ADP + 5'-phospho-(ribonucleotide)7 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | secondary structure of the ribozyme | synthetic ribozyme |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
detailed reaction kinetics, pH- and temperature-dependence | synthetic ribozyme |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00005 | - |
ATP | pH-independent, 30°C | synthetic ribozyme | |
0.00283 | - |
[gamma-S]ATP | pH-independent, 30°C | synthetic ribozyme |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
detailed reaction kinetics, pH- and temperature-dependence | synthetic ribozyme |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4-(3-bromo-8-chloro-6,11-dihydro-5H-benzo[5,6]cyclohepta[1,2-b]pyridin-11-yl)-N1-cyclohexyl-N2-[2-(1H-imidazol-4-yl)ethyl]piperazine-1,2-dicarboxamide | best phosphate-donor | synthetic ribozyme | |
ATP | - |
synthetic ribozyme |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
synthetic ribozyme | |
3.8 | - |
ADP | pH-independent in the range pH 6.0-8.5, 30°C | synthetic ribozyme |