Activating Compound | Comment | Organism | Structure |
---|---|---|---|
spermine | 1 mM enhances activity 3times | Rattus norvegicus | |
spermine | inhibition above 1 mM | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | - |
Rattus norvegicus | |
phosphate | - |
Rattus norvegicus | |
spermine | 1 mM enhances activity 3times, inhibition above 1 mM | Rattus norvegicus | |
sulfate | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
divalent cations | absolutely required | Rattus norvegicus | |
K+ | stimulates, optimal concentration: 0.1 M | Rattus norvegicus | |
Mg2+ | - |
Rattus norvegicus | |
Mn2+ | can partially replace Mg2+ in activation | Rattus norvegicus | |
Na+ | stimulates, optimal concentration: 0.1 M | Rattus norvegicus | |
NH4+ | stimulates, optimal concentration 0.1 M | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
gel filtration | Rattus norvegicus |
40000 | - |
1 * 40000, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | Rattus norvegicus | - |
ADP + 5'-phospho-DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
over 1875fold, to homogeneity | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.005 | - |
above, purified enzyme, 30°C | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, 10 mM Na2HPO4, pH 7.4, 50% v/v glycerol, 1 mM DTT, 0.001 mM ATP, stable for 2 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | - |
Rattus norvegicus | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | nuclease-treated calf thymus DNA | Rattus norvegicus | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-RNA | 5'-HO-tRNA is a very poor substrate | Rattus norvegicus | ADP + 5'-phospho-RNA | - |
? | |
ATP + synthetic oligonucleotide | (dT)10 | Rattus norvegicus | ADP + oligonucleotide 5'-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
- |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Rattus norvegicus |