Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pantoea agglomerans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
phenyl Sepharose CL-4B column chromatography | Pantoea agglomerans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.23 | - |
substrate beta-L-thymidine, pH 4.5, 37°C | Pantoea agglomerans |
0.4 | - |
substrate beta-L-2',3'-dideoxycytidine, pH 4.5, 37°C | Pantoea agglomerans |
0.55 | - |
substrate adenosine, pH 4.5, 37°C | Pantoea agglomerans |
0.57 | - |
substrate beta-L-3'-deoxy-2',3'-didehydrothymidine, pH 4.5, 37°C | Pantoea agglomerans |
0.63 | - |
substrate O2,2'-anhydro-beta-L-5-methyluridine, pH 4.5, 37°C | Pantoea agglomerans |
0.73 | - |
substrate 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl) thymine, pH 4.5, 37°C | Pantoea agglomerans |
0.95 | - |
substrate beta-L-3'-deoxy-3'-chlorothymidine, pH 4.5, 37°C | Pantoea agglomerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl) thymine | 80% maximum conversion to 5'-monophosphate | Pantoea agglomerans | 4-nitrophenol + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl) thymine 5-phosphate | - |
? | |
4-nitrophenyl phosphate + adenosine | - |
Pantoea agglomerans | 4-nitrophenol + AMP | - |
? | |
4-nitrophenyl phosphate + beta-L-2',3'-dideoxycytidine | 38% maximum conversion to 5'-monophosphate | Pantoea agglomerans | 4-nitrophenol + beta-L-2',3'-dideoxycytidine 5'-phosphate | - |
? | |
4-nitrophenyl phosphate + beta-L-3'-deoxy-2',3'-didehydrothymidine | 60% maximum conversion to 5'-monophosphate | Pantoea agglomerans | 4-nitrophenol + beta-L-3'-deoxy-2',3'-didehydrothymidine 5'-phosphate | - |
? | |
4-nitrophenyl phosphate + beta-L-3'-deoxy-3'-chlorothymidine | 90% maximum conversion to 5'-monophosphate | Pantoea agglomerans | 4-nitrophenol + beta-L-3'-deoxy-3'-chlorothymidine 5'-phosphate | - |
? | |
4-nitrophenyl phosphate + beta-L-thymidine | 35% maximum conversion to 5'-monophosphate | Pantoea agglomerans | 4-nitrophenol + beta-L-thymidine 5'-phosphate | - |
? | |
4-nitrophenyl phosphate + O2,2'-anhydro-beta-L-5-methyluridine | 65% maximum conversion to 5'-monophosphate | Pantoea agglomerans | 4-nitrophenol + O2,2'-anhydro-beta-L-5-methyluridine 5'-phosphate | - |
? | |
additional information | no activity with L-adenosine, L-cytidine, L-ribofuranosylthymine, and 1-(beta-L-arabinofuranosyl)thymine | Pantoea agglomerans | ? | - |
? | |
p-nitrophenylphosphate + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl)thymine | - |
Pantoea agglomerans | 4-nitrophenol + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl)thymine 5'-phosphate | - |
? | |
p-nitrophenylphosphate + beta-L-2',3'-dideoxycytidine | - |
Pantoea agglomerans | 4-nitrophenol + beta-L-2',3'-dideoxycytidine 5'-phosphate | - |
? | |
p-nitrophenylphosphate + L-3'-deoxy-2',3'-didehydrothymidine | - |
Pantoea agglomerans | 4-nitrophenol + L-3'-deoxy-2',3'-didehydrothymidine 5'-phosphate | - |
? | |
p-nitrophenylphosphate + L-3'-deoxy-3'-chlorothymidine | - |
Pantoea agglomerans | 4-nitrophenol + L-3'-deoxy-3'-chlorothymidine 5'-phosphate | - |
? | |
p-nitrophenylphosphate + L-thymidine | - |
Pantoea agglomerans | 4-nitrophenol + L-thymidine 5'-phosphate | - |
? | |
p-nitrophenylphosphate + O2,2'-anhydro-beta-L-5-methyluridine | - |
Pantoea agglomerans | 4-nitrophenol + O2,2'-anhydro-beta-L-5-methyluridine 5'-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NPT | - |
Pantoea agglomerans |