General Stability | Organism |
---|---|
does not lose acttivity on lyophilization | Pantoea agglomerans |
not stable to SDS | Pantoea agglomerans |
stable in presence of 2 M urea, but not 4 M urea | Pantoea agglomerans |
the enzyme can be isolated and handled only in the presence of detergents | Pantoea agglomerans |
tolerates repeated freezing and thawing | Pantoea agglomerans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Pantoea agglomerans | |
Co2+ | - |
Pantoea agglomerans | |
Cu2+ | - |
Pantoea agglomerans | |
EDTA | 1 mM, 53% inhibition of transferase activity | Pantoea agglomerans | |
Zn2+ | - |
Pantoea agglomerans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound to | Pantoea agglomerans | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM MgCl2, 20% enhancement of transferase activity with p-nitrophenol as the donor, no absolute requirement for metal ion | Pantoea agglomerans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
the purified enzyme shows two separable bands in SDS-PAGE: 70000 Da and 24000 Da | Pantoea agglomerans |
68000 | - |
sucrose density gradient sedimentation | Pantoea agglomerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pantoea agglomerans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pantoea agglomerans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
- |
Pantoea agglomerans |
Storage Stability | Organism |
---|---|
4°C, 10% glycerol, pH 7.4, stable for at least 2 months | Pantoea agglomerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-CMP + thymidine | 20% of the activity with 5'-dAMP or 5'-dTMP | Pantoea agglomerans | cytosine + TMP | - |
? | |
3'-dAMP + thymidine | 56% of the activity with 5'-dAMP or 5'-dTMP | Pantoea agglomerans | TMP + 3'-deoxyadenosine | - |
? | |
3'-TMP + thymidine | 19% of the activity with 5'-dAMP or 5'-dTMP | Pantoea agglomerans | thymidine + TMP | - |
? | |
4-nitrophenyl phosphate + 2'-deoxyadenosine | - |
Pantoea agglomerans | 2'-deoxyadenosine 5'-phosphate + 2'-deoxyadenosine 3'-phosphate + 4-nitrophenol | 5'- and 3'-nucleotide products in the approximate ratio of 2:1 | ? | |
4-nitrophenyl phosphate + adenosine | - |
Pantoea agglomerans | 5'-AMP + 4-nitrophenol | - |
? | |
4-nitrophenyl phosphate + thymidine | 24% of the activity with 5'-dAMP or 5'-dTMP | Pantoea agglomerans | 4-nitrophenol + TMP | - |
? | |
5'-AMP + thymidine | 72% of the activity with 5'-dAMP or 5'-dTMP | Pantoea agglomerans | adenosine + TMP | - |
? | |
5'-CMP + thymidine | 92% of the activity with 5'-dAMP or 5'-dTMP | Pantoea agglomerans | cytidine + TMP | - |
? | |
5'-dAMP + thymidine | - |
Pantoea agglomerans | 5'-deoxyadenosine + TMP | - |
? | |
5'-dCMP + thymidine | 46% of the activity with 5'-dAMP or 5'-dTMP | Pantoea agglomerans | 2'-deoxycytidine + TMP | - |
? | |
5'-dGMP + thymidine | 65% of the activity with 5'-dAMP or 5'-dTMP | Pantoea agglomerans | TMP + 2'-deoxyguanosine | - |
? | |
5'-dTMP + thymidine | - |
Pantoea agglomerans | 5'-deoxythymidine + TMP | - |
? | |
5'-GMP + thymidine | 61% of the activity with 5'-dAMP or 5'-dTMP | Pantoea agglomerans | TMP + guanosine | - |
? | |
adenosine 3',5'-bisphosphate + thymidine | 30% of the activity with 5'-dAMP or 5'-dTMP | Pantoea agglomerans | adenosine 3'-phosphate + TMP | - |
? | |
ADP + thymidine | 50% of the activity with 5'-dAMP or 5'-dTMP | Pantoea agglomerans | TMP + AMP | - |
? | |
ATP + thymidine | 18% of the activity with 5'-dAMP or 5'-dTMP | Pantoea agglomerans | ADP + TMP | - |
? | |
D-ribose 5-phosphate + thymidine | 13% of the activity with 5'-dAMP or 5'-dTMP | Pantoea agglomerans | D-ribose + TMP | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
5 min, stable | Pantoea agglomerans |