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Literature summary for 2.7.1.77 extracted from

  • Chao, H.M.
    Nucleoside phosphotransferase from Erwinia herbicola, a new membrane-bound enzyme (1976), J. Biol. Chem., 251, 2330-2333.
    View publication on PubMed

General Stability

General Stability Organism
does not lose acttivity on lyophilization Pantoea agglomerans
not stable to SDS Pantoea agglomerans
stable in presence of 2 M urea, but not 4 M urea Pantoea agglomerans
the enzyme can be isolated and handled only in the presence of detergents Pantoea agglomerans
tolerates repeated freezing and thawing Pantoea agglomerans

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Pantoea agglomerans
Co2+
-
Pantoea agglomerans
Cu2+
-
Pantoea agglomerans
EDTA 1 mM, 53% inhibition of transferase activity Pantoea agglomerans
Zn2+
-
Pantoea agglomerans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound to Pantoea agglomerans 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM MgCl2, 20% enhancement of transferase activity with p-nitrophenol as the donor, no absolute requirement for metal ion Pantoea agglomerans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
the purified enzyme shows two separable bands in SDS-PAGE: 70000 Da and 24000 Da Pantoea agglomerans
68000
-
sucrose density gradient sedimentation Pantoea agglomerans

Organism

Organism UniProt Comment Textmining
Pantoea agglomerans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pantoea agglomerans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
-
Pantoea agglomerans

Storage Stability

Storage Stability Organism
4°C, 10% glycerol, pH 7.4, stable for at least 2 months Pantoea agglomerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-CMP + thymidine 20% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans cytosine + TMP
-
?
3'-dAMP + thymidine 56% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans TMP + 3'-deoxyadenosine
-
?
3'-TMP + thymidine 19% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans thymidine + TMP
-
?
4-nitrophenyl phosphate + 2'-deoxyadenosine
-
Pantoea agglomerans 2'-deoxyadenosine 5'-phosphate + 2'-deoxyadenosine 3'-phosphate + 4-nitrophenol 5'- and 3'-nucleotide products in the approximate ratio of 2:1 ?
4-nitrophenyl phosphate + adenosine
-
Pantoea agglomerans 5'-AMP + 4-nitrophenol
-
?
4-nitrophenyl phosphate + thymidine 24% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans 4-nitrophenol + TMP
-
?
5'-AMP + thymidine 72% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans adenosine + TMP
-
?
5'-CMP + thymidine 92% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans cytidine + TMP
-
?
5'-dAMP + thymidine
-
Pantoea agglomerans 5'-deoxyadenosine + TMP
-
?
5'-dCMP + thymidine 46% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans 2'-deoxycytidine + TMP
-
?
5'-dGMP + thymidine 65% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans TMP + 2'-deoxyguanosine
-
?
5'-dTMP + thymidine
-
Pantoea agglomerans 5'-deoxythymidine + TMP
-
?
5'-GMP + thymidine 61% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans TMP + guanosine
-
?
adenosine 3',5'-bisphosphate + thymidine 30% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans adenosine 3'-phosphate + TMP
-
?
ADP + thymidine 50% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans TMP + AMP
-
?
ATP + thymidine 18% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans ADP + TMP
-
?
D-ribose 5-phosphate + thymidine 13% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans D-ribose + TMP
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
5 min, stable Pantoea agglomerans