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Literature summary for 2.7.1.77 extracted from

  • Rodgers, R.; Chargaff, E.
    Nucleoside phosphotransferase from carrot. Chemical characterization and investigation of catalytic sites (1972), J. Biol. Chem., 247, 5448-5455.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
2,4-dinitrofluorobenzene
-
 Daucus carota
Iodine
-
 Daucus carota
Urea 6 M, complete inactivation Daucus carota

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 gel filtration, equilibrium sedimentation, sucrose density gradient centrifugation Daucus carota

Organism

Organism UniProt Comment Textmining
Daucus carota
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Daucus carota

Subunits

Subunits Comment Organism
dimer 1 * 21500-25000 + 1 * 21500-25000, subunits differ substantially in amino acid composition, SDS-PAGE, sedimentation equilibrium ultracentrifugation or sucrose density gradient centrifugation in presence of 6 M guanidine HCl Daucus carota

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Daucus carota

pH Stability

pH Stability pH Stability Maximum Comment Organism
9.5
-
 without protecting agent, about one-third of the initial transfer activity is retained whereas the hydrolase function is almost entirely lost after 22 h Daucus carota

pI Value

Organism Comment pI Value Maximum pI Value
Daucus carota isoelectric focusing
-
 4.1
Daucus carota isoelectric focusing 4.7 4.6
Daucus carota isoelectric focusing
-
 5.1