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Literature summary for 2.7.1.77 extracted from
- Nucleoside phosphotransferase from carrot (1978), Methods Enzymol., 51, 387-394.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NaF | 0.25 M, enhances activity by about 45% | Daucus carota |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Daucus carota | |
| cysteine | - |
Daucus carota | |
| EDTA | - |
Daucus carota | |
| Fe2+ | - |
Daucus carota | |
| Fe3+ | - |
Daucus carota | |
| glutathione | - |
Daucus carota | |
| Iodine | - |
Daucus carota | |
| Mn2+ | - |
Daucus carota | |
| NaF | 0.25 M, phosphatase is inhibited by less than 10% | Daucus carota | |
| Pb2+ | - |
Daucus carota | |
| uridine | inhibitor of phosphatase reaction | Daucus carota | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
Phenylphosphate | phosphotransferase reaction with uridine | Daucus carota | |
| 3.5 | - |
uridine | phosphotransferase reaction with phenyl phosphate | Daucus carota | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
gel filtration, equilibrium sedimentation, sucrose density gradient centrifugation | Daucus carota |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Daucus carota | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Daucus carota |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Daucus carota | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 32 | - |
- |
Daucus carota |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 1 months | Daucus carota |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | - |
Daucus carota | ? | - |
? | |
| phenyl phosphate + adenosine | - |
Daucus carota | AMP + phenol | - |
? | |
| phenyl phosphate + cytidine | - |
Daucus carota | CMP + phenol | - |
? | |
| phenyl phosphate + deoxyadenosine | - |
Daucus carota | dAMP + phenol | - |
? | |
| phenyl phosphate + deoxycytidine | - |
Daucus carota | dCMP + phenol | - |
? | |
| phenyl phosphate + deoxyguanosine | - |
Daucus carota | dGMP + phenol | - |
? | |
| phenyl phosphate + deoxyuridine | - |
Daucus carota | dUMP + phenol | - |
? | |
| phenyl phosphate + guanosine | - |
Daucus carota | GMP + phenol | - |
? | |
| phenyl phosphate + thymidine | - |
Daucus carota | TMP + phenol | - |
? | |
| phenyl phosphate + uridine | - |
Daucus carota | 5'-UMP + phenol | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Daucus carota |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
without protecting agent, about one-third of the initial transfer activity is retained whereas the hydrolase function is almost entirely lost after 22 h | Daucus carota |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Daucus carota | intact enzyme | - |
4.1 |
| Daucus carota | subunit A | 4.7 | 4.6 |
| Daucus carota | subunit B | - |
5.1 |
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Release 2023.1 (January 2023)
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