Application | Comment | Organism |
---|---|---|
medicine | ability of the designer enzymes to activate 5-substitued pyrimidines makes it possible to utilize such nucleoside analogs in suicide gene therapy or protein therapy applications that target cancer cells | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain C41(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinanat Wild-type enzyme in complex with 5-methyldeoxycytidine and ADP, hanging drop vapour diffusion method, mixing of 0.001 ml of 8 mg/ml protein mixed with 0.001 ml reservoir solution containing 0.9-1.5 M trisodium citrate dihydrate and 100 mM Tris, pH 7.5, room temperature, X-ray diffraction structure determination and analysis at 1.96 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R104I/D133A | site-directed mutagenesis, the mutations render the enzyme active with 5-substituted deoxycytidine and thymidine, altered substrate specificity compared to the wild-type enzyme | Homo sapiens |
R104L/D133A | site-directed mutagenesis, the mutations render the enzyme active with 5-substituted deoxycytidine and thymidine, altered substrate specificity compared to the wild-type enzyme | Homo sapiens |
R104M/D133A | site-directed mutagenesis, the mutations render the enzyme active with 5-substituted deoxycytidine and thymidine, altered substrate specificity compared to the wild-type enzyme | Homo sapiens |
R104Q/D133A | site-directed mutagenesis, the mutations render the enzyme active with 5-substituted deoxycytidine and thymidine, altered substrate specificity compared to the wild-type enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-methyldeoxycytidine | Homo sapiens | - |
ADP + 5-methyl-dCMP | - |
? | |
ATP + deoxycytidine | Homo sapiens | - |
ADP + dCMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27707 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain C41(DE3) by nickel affinity chromatography and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine | substrate of enzyme mutant R104M/D133A, poor substrate of the wild-type enzyme | Homo sapiens | ADP + ? | - |
? | |
ATP + 5-bromodeoxycytidine | - |
Homo sapiens | ADP + 5-bromo-dCMP | - |
? | |
ATP + 5-iododeoxycytidine | - |
Homo sapiens | ADP + 5-iodo-dCMP | - |
? | |
ATP + 5-methyldeoxycytidine | - |
Homo sapiens | ADP + 5-methyl-dCMP | - |
? | |
ATP + 5-propynyldeoxycytidine | - |
Homo sapiens | ADP + 5-propynyl-dCMP | - |
? | |
ATP + D-deoxycytidine | - |
Homo sapiens | ADP + D-dCMP | - |
? | |
ATP + D-deoxythymidine | - |
Homo sapiens | ADP + D-dTMP | - |
? | |
ATP + D-deoxyuridine | - |
Homo sapiens | ADP + D-dUMP | - |
? | |
ATP + deoxycytidine | - |
Homo sapiens | ADP + dCMP | - |
? | |
ATP + gemcitabine | - |
Homo sapiens | ADP + phospho-gemcitabine | - |
? | |
ATP + L-deoxyuridine | - |
Homo sapiens | ADP + L-dUMP | - |
? | |
ATP + L-thymidine | - |
Homo sapiens | ADP + L-dTMP | - |
? | |
additional information | substrate specificity or wild-type an dmutant enzymes, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dCK | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | structure-function analysis and substrate specificity, overview | Homo sapiens |