Crystallization (Comment) | Organism |
---|---|
structural studies of ternary complexes. The enzyme conformation adjusts to the different hydrogen-bonding properties between 2'-deoxyadenosine and 2'-deoxyguanosine and to the presence of substituent at the 2-position present in 2'-deoxyguanosine and cladribine. The carbonyl group in 2'-deoxyguansoine elicits a conformational adjustment of the active site residues Arg104 and Asp133. 2'-Deoxyguanosine and cladribine adopt the anti conformation, in contrast to the syn conformation observed with 2'-deoxyadenosine | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0051 | - |
2-chlorodeoxyadenosine | cosubstrate UTP, pH 7.5, 37°C | Homo sapiens | |
0.0085 | - |
2'-deoxyadenosine | cosubstrate UTP, pH 7.5, 37°C | Homo sapiens | |
0.041 | - |
2'-deoxyguanosine | cosubstrate UTP, pH 7.5, 37°C | Homo sapiens | |
0.078 | - |
2-chlorodeoxyadenosine | cosubstrate ATP, pH 7.5, 37°C | Homo sapiens | |
0.115 | - |
2'-deoxyadenosine | cosubstrate ATP, pH 7.5, 37°C | Homo sapiens | |
0.181 | - |
2'-deoxyguanosine | cosubstrate ATP, pH 7.5, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27707 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxyadenosine | - |
Homo sapiens | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxyguanosine | - |
Homo sapiens | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
ATP + 2-chlorodeoxyadenosine | i.e. cladribine | Homo sapiens | ADP + 2-chlorodeoxyadenosine 5'-phosphate | - |
? | |
UTP + 2'-deoxyadenosine | - |
Homo sapiens | UDP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
UTP + 2'-deoxyguanosine | - |
Homo sapiens | UDP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
UTP + 2-chlorodeoxyadenosine | i.e. cladribine | Homo sapiens | UDP + 2-chlorodeoxyadenosine 5'-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
2'-deoxyadenosine | cosubstrate UTP, pH 7.5, 37°C | Homo sapiens | |
0.37 | - |
2-chlorodeoxyadenosine | cosubstrate UTP, pH 7.5, 37°C | Homo sapiens | |
0.53 | - |
2'-deoxyguanosine | cosubstrate UTP, pH 7.5, 37°C | Homo sapiens | |
0.6 | - |
2-chlorodeoxyadenosine | cosubstrate ATP, pH 7.5, 37°C | Homo sapiens | |
2.1 | - |
2'-deoxyadenosine | cosubstrate ATP, pH 7.5, 37°C | Homo sapiens | |
2.5 | - |
2'-deoxyguanosine | cosubstrate ATP, pH 7.5, 37°C | Homo sapiens |