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Literature summary for 2.7.1.74 extracted from

  • Smal, C.; Vertommen, D.; Bertrand, L.; Ntamashimikiro, S.; Rider, M.H.; Van Den Neste, E.; Bontemps, F.
    Identification of in vivo phosphorylation sites on human deoxycytidine kinase. Role of Ser-74 in the control of enzyme activity (2006), J. Biol. Chem., 281, 4887-4893.
    View publication on PubMed
Search for more literature for 2.7.1.74

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag fusion protein in HEK293T cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
S11A no significant effect on activity Homo sapiens
S11E no significant effect on activity Homo sapiens
S15A no significant effect on activity Homo sapiens
S15E no significant effect on activity Homo sapiens
S74A mutation markedly decreases activity Homo sapiens
S74E mutation does not significantly modify dCK activity Homo sapiens
T3A no significant effect on activity Homo sapiens
T3E no significant effect on activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
lambda protein phosphatase
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00016
-
 2'-deoxycytidine with ATP as cosubstrate Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 2 * 34000, SDS-PAGE, native mass by gel filtration Homo sapiens
68000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2'-deoxyadenosine Homo sapiens rate-limiting reaction in the salvage of deoxyribonucleosides ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
ATP + 2'-deoxycytidine Homo sapiens rate-limiting reaction in the salvage of deoxyribonucleosides ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
ATP + 2'-deoxyguanosine Homo sapiens rate-limiting reaction in the salvage of deoxyribonucleosides ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
additional information Homo sapiens catalyzes the rate-limiting step of the deoxyribonucleoside salvage pathway, key role in the activation of numerous nucleoside analogues used in anti-cancer and antiviral chemotherapy ?
-
 ?
NTP + 1-beta-D-arabinosylcytosine Homo sapiens i.e. cytaribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies NDP + 1-beta-D-arabinosylcytosine 5'-phosphate
-
 ?
NTP + 2',2'-difluorodeoxycytidine Homo sapiens i.e. gemcitabine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of solid malignant tumors NDP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
 ?
NTP + 2',3'-dideoxycytidine Homo sapiens i.e. zalcitabine, activation of the pharmaceutically relevant substrate which is used in the treatment of HIV infections NDP + 2',3'-dideoxycytidine 5'-phosphate
-
 ?
NTP + 2'-deoxy-3'-thiacytidine Homo sapiens i.e. lamivudine, activation of the pharmaceutically relevant substrate which is used in the treatment of HIV infections NDP + 2'-deoxy-3'-thiacytidine 5'-phosphate
-
 ?
NTP + 2-chloro-2'-deoxyadenosine Homo sapiens i.e. cladribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
 ?
NTP + 9-beta-D-arabinosyl 2-fluoroadenine Homo sapiens i.e. fludarabine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies NDP + 9-beta-D-arabinosyl 2-fluoroadenine 5'-phosphate
-
 ?
UTP + 2'-deoxyadenosine Homo sapiens rate-limiting reaction in the salvage of deoxyribonucleosides UDP + 2'-deoxyadenosine 5'-phosphate
-
 ?
UTP + 2'-deoxycytidine Homo sapiens rate-limiting reaction in the salvage of deoxyribonucleosides UDP + 2'-deoxycytidine 5'-phosphate
-
 ?
UTP + 2'-deoxyguanosine Homo sapiens rate-limiting reaction in the salvage of deoxyribonucleosides UDP + 2'-deoxyguanosine 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P27707
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein at least four residues phosphorylated, Thr-3, Ser-11, Ser-15, and Ser-74, Ser-74 phosphorylation level important for enzyme activity Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
CCRF-CEM cell
-
 Homo sapiens
-
leukemia cell
-
 Homo sapiens
-
lymphoid cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxyadenosine rate-limiting reaction in the salvage of deoxyribonucleosides Homo sapiens ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
ATP + 2'-deoxycytidine rate-limiting reaction in the salvage of deoxyribonucleosides Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
ATP + 2'-deoxyguanosine rate-limiting reaction in the salvage of deoxyribonucleosides Homo sapiens ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
additional information catalyzes the rate-limiting step of the deoxyribonucleoside salvage pathway, key role in the activation of numerous nucleoside analogues used in anti-cancer and antiviral chemotherapy Homo sapiens ?
-
 ?
NTP + 1-beta-D-arabinosylcytosine i.e. cytaribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies Homo sapiens NDP + 1-beta-D-arabinosylcytosine 5'-phosphate
-
 ?
NTP + 2',2'-difluorodeoxycytidine i.e. gemcitabine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of solid malignant tumors Homo sapiens NDP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
 ?
NTP + 2',3'-dideoxycytidine i.e. zalcitabine, activation of the pharmaceutically relevant substrate which is used in the treatment of HIV infections Homo sapiens NDP + 2',3'-dideoxycytidine 5'-phosphate
-
 ?
NTP + 2'-deoxy-3'-thiacytidine i.e. lamivudine, activation of the pharmaceutically relevant substrate which is used in the treatment of HIV infections Homo sapiens NDP + 2'-deoxy-3'-thiacytidine 5'-phosphate
-
 ?
NTP + 2-chloro-2'-deoxyadenosine i.e. cladribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies Homo sapiens NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
 ?
NTP + 9-beta-D-arabinosyl 2-fluoroadenine i.e. fludarabine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies Homo sapiens NDP + 9-beta-D-arabinosyl 2-fluoroadenine 5'-phosphate
-
 ?
UTP + 2'-deoxyadenosine rate-limiting reaction in the salvage of deoxyribonucleosides Homo sapiens UDP + 2'-deoxyadenosine 5'-phosphate
-
 ?
UTP + 2'-deoxycytidine rate-limiting reaction in the salvage of deoxyribonucleosides Homo sapiens UDP + 2'-deoxycytidine 5'-phosphate
-
 ?
UTP + 2'-deoxyguanosine rate-limiting reaction in the salvage of deoxyribonucleosides Homo sapiens UDP + 2'-deoxyguanosine 5'-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE, native mass by gel filtration Homo sapiens

Synonyms

Synonyms Comment Organism
dCK
-
 Homo sapiens