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Literature summary for 2.7.1.74 extracted from
- Mammalian deoxynucleoside kinase. I. Deoxycytidine kinase: purification, properties, and kinetic studies with cytosine arabinoside (1968), J. Biol. Chem., 243, 4298-4304.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | not | Bos taurus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cytosine arabinoside | deoxycytidine as substrate | Bos taurus | |
| dCDP | - |
Bos taurus | |
| dCMP | - |
Bos taurus | |
| dCTP | - |
Bos taurus | |
| deoxyadenosine | not | Bos taurus | |
| deoxycytidine | cytosine arabinoside as substrate | Bos taurus | |
| deoxyguanosine | not | Bos taurus | |
| HgCl2 | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
deoxycytidine | 37°C, pH 8.0 | Bos taurus | |
| 0.014 | 0.0167 | deoxycytidine | 37°C, pH 8.0 | Bos taurus | |
| 0.02 | 0.04 | cytosine arabinoside | 37°C, pH 8.0 | Bos taurus | |
| 0.11 | - |
ATP | cosubstrate deoxycytidine, 37°C, pH 8.0 | Bos taurus | |
| 0.2 | - |
ATP | cosubstrate cytosine arabinoside, 37°C, pH 8.0 | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Bos taurus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation | Bos taurus | |
| Ca2+ | can replace Mn2+ or Mg2+ to some extent | Bos taurus | |
| Mg2+ | more efficient than Mn2+ or Ca2+ | Bos taurus | |
| Mn2+ | requirement | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thymus | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00632 | - |
37°C, pH 8.0 | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2'-deoxycytidine | - |
Bos taurus | ADP + 5'-dCMP | - |
? | |
| CTP + 2'-deoxycytidine | - |
Bos taurus | CDP + 2'-deoxy-CMP | - |
? | |
| dGTP + 2'-deoxycytidine | - |
Bos taurus | dGDP + 2'-deoxy-CMP | - |
? | |
| dTTP + 2'-deoxycytidine | - |
Bos taurus | dTDP + 2'-deoxy-CMP | - |
? | |
| GTP + 2'-deoxycytidine | best phosphate donor | Bos taurus | GDP + 2'-deoxy-CMP | - |
? | |
| NTP + cytosine arabinoside | ATP can be replaced by GTP, dATP, dGTP or dTTP | Bos taurus | NDP + cytosine arabinoside 5'-monophosphate | - |
? | |
| NTP + deoxycytidine | enzyme shows little specificity towards phosphate donor | Bos taurus | NDP + dCMP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
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