Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Polyphosphate | dependent on, can be substituted by ATP, nonprocessive utilization, binding site is the same as for ATP binding | Arthrobacter sp. |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis | Arthrobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
Hexametaphosphate | pH 7.0 | Arthrobacter sp. | |
0.2 | - |
ATP | pH 7.0 | Arthrobacter sp. | |
0.5 | - |
D-glucose | pH 7.0 | Arthrobacter sp. | |
15 | - |
D-mannose | pH 7.0 | Arthrobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates, 86% and 30% activity with polyphosphate and ATP, respectively, compared to Mg2+ | Arthrobacter sp. | |
Cu2+ | activates, 11% activity with polyphosphate compared to Mg2+, no activity with ATP | Arthrobacter sp. | |
Fe2+ | activates, 15% activity with ATP compared to Mg2+, no activity with polyphosphate | Arthrobacter sp. | |
Mg2+ | absolutely required, most effective metal ion | Arthrobacter sp. | |
Mn2+ | activates, 82% and 88% activity with polyphosphate and ATP, respectively, compared to Mg2+ | Arthrobacter sp. | |
additional information | activities with different divalent cations in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, and Fe2+, no activity with Hg2+ | Arthrobacter sp. | |
Zn2+ | activates, 80% and 19% activity with polyphosphate and ATP, respectively, compared to Mg2+ | Arthrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
- |
Arthrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | Q7WT42 | - |
- |
Arthrobacter sp. KM | Q7WT42 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 2200fold and 1375fold determined with polyphosphate and ATP, respectively, to homogeneity | Arthrobacter sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + D-mannose = ADP + D-mannose 6-phosphate | mechanism, enzyme shows both mannokinase and glucokinase activities | Arthrobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.064 | - |
mannokinase activity with polyphosphate in cell extract | Arthrobacter sp. |
0.077 | - |
mannokinase activity with ATP in cell extract | Arthrobacter sp. |
110 | - |
purified enzyme, mannokinase activity with ATP | Arthrobacter sp. |
220 | - |
purified enzyme, mannokinase activity with polyphosphate | Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Arthrobacter sp. | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | - |
Arthrobacter sp. KM | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-mannose | - |
Arthrobacter sp. | ADP + D-mannose 6-phosphate | - |
? | |
ATP + D-mannose | - |
Arthrobacter sp. KM | ADP + D-mannose 6-phosphate | - |
? | |
hexametaphosphate + D-mannose | - |
Arthrobacter sp. | pentaphosphate + D-mannose 6-phosphate | - |
? | |
hexametaphosphate + D-mannose | - |
Arthrobacter sp. KM | pentaphosphate + D-mannose 6-phosphate | - |
? | |
additional information | enzyme is also active with D-glucose, which is a more efficient substrate than D-mannose, ratio 3:2 and 3:1 with ATP and polyphosphate, respectively | Arthrobacter sp. | ? | - |
? | |
additional information | enzyme is also active with D-glucose, which is a more efficient substrate than D-mannose, ratio 3:2 and 3:1 with ATP and polyphosphate, respectively | Arthrobacter sp. KM | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000 | Arthrobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
inorganic polyphosphate/ATP-glucomannokinase | - |
Arthrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Arthrobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
loss of 50% activity after 5 min | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Arthrobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on, can be substituted by polyphosphate, binding site is the same as for polyphosphate binding | Arthrobacter sp. |