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Literature summary for 2.7.1.7 extracted from

  • Mukai, T.; Kawai, S.; Matsukawa, H.; Matuo, Y.; Murata, K.
    Characterization and molecular cloning of a novel enzyme, inorganic polyphosphate/ATP-glucomannokinase, of Arthrobacter sp. strain KM (2003), Appl. Environ. Microbiol., 69, 3849-3857.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.7

Activating Compound

Activating Compound Comment Organism Structure
Polyphosphate dependent on, can be substituted by ATP, nonprocessive utilization, binding site is the same as for ATP binding Arthrobacter sp.

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 Hexametaphosphate pH 7.0 Arthrobacter sp.
0.2
-
 ATP pH 7.0 Arthrobacter sp.
0.5
-
 D-glucose pH 7.0 Arthrobacter sp.
15
-
 D-mannose pH 7.0 Arthrobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates, 86% and 30% activity with polyphosphate and ATP, respectively, compared to Mg2+ Arthrobacter sp.
Cu2+ activates, 11% activity with polyphosphate compared to Mg2+, no activity with ATP Arthrobacter sp.
Fe2+ activates, 15% activity with ATP compared to Mg2+, no activity with polyphosphate Arthrobacter sp.
Mg2+ absolutely required, most effective metal ion Arthrobacter sp.
Mn2+ activates, 82% and 88% activity with polyphosphate and ATP, respectively, compared to Mg2+ Arthrobacter sp.
additional information activities with different divalent cations in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, and Fe2+, no activity with Hg2+ Arthrobacter sp.
Zn2+ activates, 80% and 19% activity with polyphosphate and ATP, respectively, compared to Mg2+ Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
-
 Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. Q7WT42
-
-
Arthrobacter sp. KM Q7WT42
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 2200fold and 1375fold determined with polyphosphate and ATP, respectively, to homogeneity Arthrobacter sp.

Reaction

Reaction Comment Organism Reaction ID
ATP + D-mannose = ADP + D-mannose 6-phosphate mechanism, enzyme shows both mannokinase and glucokinase activities Arthrobacter sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.064
-
 mannokinase activity with polyphosphate in cell extract Arthrobacter sp.
0.077
-
 mannokinase activity with ATP in cell extract Arthrobacter sp.
110
-
 purified enzyme, mannokinase activity with ATP Arthrobacter sp.
220
-
 purified enzyme, mannokinase activity with polyphosphate Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
-
 Arthrobacter sp. ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose
-
 Arthrobacter sp. KM ADP + D-glucose 6-phosphate
-
 ?
ATP + D-mannose
-
 Arthrobacter sp. ADP + D-mannose 6-phosphate
-
 ?
ATP + D-mannose
-
 Arthrobacter sp. KM ADP + D-mannose 6-phosphate
-
 ?
hexametaphosphate + D-mannose
-
 Arthrobacter sp. pentaphosphate + D-mannose 6-phosphate
-
 ?
hexametaphosphate + D-mannose
-
 Arthrobacter sp. KM pentaphosphate + D-mannose 6-phosphate
-
 ?
additional information enzyme is also active with D-glucose, which is a more efficient substrate than D-mannose, ratio 3:2 and 3:1 with ATP and polyphosphate, respectively Arthrobacter sp. ?
-
 ?
additional information enzyme is also active with D-glucose, which is a more efficient substrate than D-mannose, ratio 3:2 and 3:1 with ATP and polyphosphate, respectively Arthrobacter sp. KM ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30000 Arthrobacter sp.

Synonyms

Synonyms Comment Organism
inorganic polyphosphate/ATP-glucomannokinase
-
 Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 loss of 50% activity after 5 min Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Arthrobacter sp.

Cofactor

Cofactor Comment Organism Structure
ATP dependent on, can be substituted by polyphosphate, binding site is the same as for polyphosphate binding Arthrobacter sp.