Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,2-diarachidonoyl phosphatidic acid | - |
Mus musculus | |
1,2-dilinoleoyl phosphatidic acid | - |
Mus musculus | |
1,2-dioleoyl phosphatidic acid | - |
Mus musculus | |
1-arachidoyl-2-arachidonoyl phosphatidic acid | - |
Mus musculus | |
1-stearoyl-2-arachidonoyl phosphatidic acid | - |
Mus musculus | |
1-stearoyl-2-oleoyl phosphatidic acid | - |
Mus musculus | |
additional information | PIP5K activation depends on the acyl chain composition of both substrate and activator | Mus musculus | |
phosphatidic acid | - |
Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression of HA-tagged PIP5K isozymes in COS-7 cells, expression of isozyme PIP5Kalpha mutant D322A | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D322A | isozyme PIP5Kalpha mutant that shows reduced activity compared to the wild-type enzyme | Mus musculus |
L202I | the mutations of PIP5Kalpha decreases the substrate affinity and the enzyme efficiency for 1-stearoyl-2-arachidonoyl phosphatidylinositol-4-phosphate compared to the wild-type enzyme | Mus musculus |
L210I | the mutations of PIP5Kalpha decreases the substrate affinity and the enzyme efficiency for 1-stearoyl-2-arachidonoyl phosphatidylinositol-4-phosphate compared to the wild-type enzyme | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4-phosphate | isozyme PIP5Kgamma, pH 7.5, temperature not specified in the publication | Mus musculus | |
2.8 | - |
1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4-phosphate | isozyme PIP5Kalpha, pH 7.5, temperature not specified in the publication | Mus musculus | |
3.7 | - |
1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4-phosphate | isozyme PIP5Kbeta, pH 7.5, temperature not specified in the publication | Mus musculus | |
4.9 | - |
1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4-phosphate | isozyme PIP5Kbeta, pH 7.5, temperature not specified in the publication | Mus musculus | |
15 | - |
1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4-phosphate | isozyme PIP5Kgamma, pH 7.5, temperature not specified in the publication | Mus musculus | |
16 | - |
1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4-phosphate | isozyme PIP5Kalpha, pH 7.5, temperature not specified in the publication | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | isozyme PIP5Kalpha | Mus musculus | 16020 | - |
nucleus | isozyme PIP5Kalpha | Mus musculus | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | Mus musculus | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-dipalmitoyl phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
Mus musculus | ADP + 1,2-dipalmitoyl phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Mus musculus | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4-phosphate | - |
Mus musculus | ADP + 1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4-phosphate | - |
Mus musculus | ADP + 1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphatidylinositol-4-phosphate 5-kinase | - |
Mus musculus |
PIP5K | - |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |