Literature summary for 2.7.1.68 extracted from

Padron, D.; Wang, Y.J.; Yamamoto, M.; Yin, H.; Roth, M.G.
Phosphatidylinositol phosphate 5-kinase Ibeta recruits AP-2 to the plasma membrane and regulates rates of constitutive endocytosis (2003), J. Cell Biol., 162, 693-701.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of isozymes alpha, beta, and gamma in HeLa cells, overexpression of isozymes alpha, beta, and gamma in CV-1 cells: isozymes alpha and beta are active, the latter shows the highest activity, isozyme gamma is not active, small interference RNA inhibition of isozyme beta expression also reduces expression of isozyme alpha, but increased expression of isozyme gamma, with a 50% loss in activity, reduction of isozyme alpha expression increases expression of isozymes beta and gamma and leaves the 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate synthesis level unaltered, inhibition of isozyme gamma increases expression of isozymes alpha and beta, overview, expression of isozyme beta increases the internalization of influenza virus hemagglutinin Y543, enzyme expression prevents the cells of disruption of their actine cytoskeleton by drugs, e.g. cytochalasin D or latrunculin, and increases the number of clathrin-coated pits	Mus musculus

Cloned (Comment)

Organism

overexpression of isozymes alpha, beta, and gamma in HeLa cells, overexpression of isozymes alpha, beta, and gamma in CV-1 cells: isozymes alpha and beta are active, the latter shows the highest activity, isozyme gamma is not active, small interference RNA inhibition of isozyme beta expression also reduces expression of isozyme alpha, but increased expression of isozyme gamma, with a 50% loss in activity, reduction of isozyme alpha expression increases expression of isozymes beta and gamma and leaves the 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate synthesis level unaltered, inhibition of isozyme gamma increases expression of isozymes alpha and beta, overview, expression of isozyme beta increases the internalization of influenza virus hemagglutinin Y543, enzyme expression prevents the cells of disruption of their actine cytoskeleton by drugs, e.g. cytochalasin D or latrunculin, and increases the number of clathrin-coated pits

Mus musculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate	Mus musculus	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate, especially produced by isozyme beta, is required for constitutive endocytosis of transferrin receptors via clathrin-coated pits, and probably also for the regulation thereof	ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	3 isozymes alpha, beta, and gamma	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate	-	Mus musculus	ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate	-	?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate, especially produced by isozyme beta, is required for constitutive endocytosis of transferrin receptors via clathrin-coated pits, and probably also for the regulation thereof	Mus musculus	ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate	-	?

Synonyms

Synonyms	Comment	Organism
phosphatidylinositol phosphate 5-kinase Ibeta	-	Mus musculus
PIP5KI	-	Mus musculus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Mus musculus