Cloned (Comment) | Organism |
---|---|
identification of splicing form c of type I phosphatidylinositol 4-phosphate 5-kinase isoform gamma possessing additional 26 amino acids at the C-terminus from a hippocampus library, DNA and amino acid sequence determination and analysis, overvexpression of the catalytically inactive mutants D316K and K188A of isozyme gamma splicing form c in rat cerebellar granule cells, expression of isozyme gamma splicing form a, expression of wild-type and FLAG-tagged isozyme gamma splicing form c in COS-7 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D316K | site-directed mutagenesis, overexpression of the catalytically inactive construct of isozyme gamma splicing form c in rat cerebellar granule cells causes progressive loss of their neuronal processes, not observed with expression of isozyme gamma splicing form a | Rattus norvegicus |
K188A | site-directed mutagenesis, overexpression of the catalytically inactive construct of isozyme gamma splicing form c in rat cerebellar granule cells causes progressive loss of their neuronal processes, not observed with expression of isozyme gamma splicing form a | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | at 80 mM | Rattus norvegicus | |
MgCl2 | at 10 mM | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75600 | - |
x * 75600, isozyme splicing form c, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | Rattus norvegicus | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
additional information | Rattus norvegicus | splicing form a of the isozyme gamma is inactive, splicing form c is involved in maintenance of some neuronal cellular processes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
type I phosphatidylinositol 4-phosphate 5-kinase isozyme gamma, 3 splicing forms a-c | - |
Purification (Comment) | Organism |
---|---|
wild-type and mutant isozyme gamma, partially by immunoprecipitation and protein G affinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
cerebellum | granule cells | Rattus norvegicus | - |
hippocampus | dentate gyrus, pyramidal cells | Rattus norvegicus | - |
neuron | in the pyramidal cells of the hippocampus and of the cerebral cortex, splicing form c of isozyme gamma is neuronal-specific | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Rattus norvegicus | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
additional information | splicing form a of the isozyme gamma is inactive, splicing form c is involved in maintenance of some neuronal cellular processes | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75600, isozyme splicing form c, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
phosphatidylinositol 4-phosphate 5-kinase | - |
Rattus norvegicus |
PIPkin | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |