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Literature summary for 2.7.1.68 extracted from

  • Vancurova, I.; Choi, J.H.; Lin, H.; Kuret, J.; Vancura, A.
    Regulation of phosphatidylinositol 4-phosphate 5-kinase from Schizosaccharomyces pombe by casein kinase I (1999), J. Biol. Chem., 274, 1147-1155.
    View publication on PubMed
Search for more literature for 2.7.1.68

Activating Compound

Activating Compound Comment Organism Structure
spermine 0.5 mM, 300% increase in activity Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate 0.1 mM, 55% inhibition Schizosaccharomyces pombe
phosphatidic acid 0.1 mM, 125% stimulation Schizosaccharomyces pombe
phosphatidylinositol 0.1 mM, 125% stimulation Schizosaccharomyces pombe
phosphatidylserine 0.1 mM, 125% stimulation Schizosaccharomyces pombe
spermine 0.03 mg/ml, 50% inhibition Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for 1-phosphatidyl-1D-myo-inositol 4-phosphate is 1.0 mol% Schizosaccharomyces pombe
0.008
-
 ATP pH 7.5 Schizosaccharomyces pombe

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Schizosaccharomyces pombe 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 x * 63000, SDS-PAGE Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate Schizosaccharomyces pombe the enzyme catalyzes the last step in the synthesis of phosphatidylinositol 4,5-bisphosphate, which is a precursor of diacylglycerol and inositol 1,4,5-triphosphate and is also involved in regulation of actin cytoskeleton remodeling and membrane traffic. The enzyme is regulated by casein kinase Cki1 ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation by Cki1 in vitro decreases the activity of the enzyme Schizosaccharomyces pombe

Purification (Commentary)

Purification (Comment) Organism
-
 Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.704
-
-
 Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Schizosaccharomyces pombe ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate the enzyme catalyzes the last step in the synthesis of phosphatidylinositol 4,5-bisphosphate, which is a precursor of diacylglycerol and inositol 1,4,5-triphosphate and is also involved in regulation of actin cytoskeleton remodeling and membrane traffic. The enzyme is regulated by casein kinase Cki1 Schizosaccharomyces pombe ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 63000, SDS-PAGE Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Schizosaccharomyces pombe

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 pH 5.5: about 35% of maximal activity, pH 8.5: about 60% of maximal activity Schizosaccharomyces pombe