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Literature summary for 2.7.1.68 extracted from

  • Ling, L.E.; Schulz, J.T.; Cantley, L.C.
    Characterization and purification of membrane-associated phosphatidylinositol-4-phosphate kinase from human red blood cells (1989), J. Biol. Chem., 264, 5080-5088.
    View publication on PubMed
Search for more literature for 2.7.1.68

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 the optimal activity obtained in Triton X-100 is 6fold higher than that obtained in the absence of Triton X-100 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate half-maximal inhibition at a ratio of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1-phosphatidyl-1D-myo-inositol 4-phosphate of 1.5:1 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 ATP pH 7.4, 23-25°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated with, peripheral membrane protein Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Km: 2 mM, Mg2+ is three times more effective than Mn2+ Homo sapiens
Mn2+ Km: 0.2 mM, Mg2+ is three times more effective than Mn2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 x * 53000, SDS-PAGE Homo sapiens
150000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.117
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, stable in concentrated form Homo sapiens
4°C, storage causes the 53000 Da enzyme to be slowly degraded to a 45000 Da peptide Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Homo sapiens