Activating Compound | Comment | Organism | Structure |
---|---|---|---|
spermine | strong activation in presence of micromolar concentrations, at 2 mM Mg2+ optimal spermine concentration is 0.1-0.2 mM spermine | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
heparin | IC50: 0.002 mg/ml, competitive towards 1-phosphatidyl-1D-myo-inositol 4-phosphate | Rattus norvegicus | |
quercetin | IC50: 0.0002 mM, competitive towards ATP or GTP | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-value for 1-phosphatidyl-1D-myo-inositol 4-phosphate is 0.0033 mg/ml | Rattus norvegicus | |
0.025 | - |
ATP | pH 7.5, 22°C | Rattus norvegicus | |
0.133 | - |
GTP | pH 7.5, 22°C | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimal concentration is 20-30 mM | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, stable for at least 3 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Rattus norvegicus | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
GTP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | 50% of the activity with ATP | Rattus norvegicus | GDP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? |