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Literature summary for 2.7.1.67 extracted from

  • Baumlova, A.; Chalupska, D.; Rozycki, B.; Jovic, M.; Wisniewski, E.; Klima, M.; Dubankova, A.; Kloer, D.P.; Nencka, R.; Balla, T.; Boura, E.
    The crystal structure of the phosphatidylinositol 4-kinase IIalpha (2014), EMBO Rep., 15, 1085-1092.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
phosphatidylinositol 4-kinase type IIalpha in complex with ATP, vapor diffusion method Homo sapiens

Protein Variants

Protein Variants Comment Organism
K165-K172 when the entire membrane binding segment K165-K172 was mutated to alanines, the kinase is inactive Homo sapiens
N163A the mutant shows about 10% of wild type activity Homo sapiens
N249A the mutant shows slightly reduced wild type activity Homo sapiens
R275A the mutant shows about 15% of wild type activity Homo sapiens
V339A the mutant shows slightly reduced wild type activity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol Homo sapiens
-
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9BTU6
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity, anion exchange column chromatography, and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol
-
Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
?

Synonyms

Synonyms Comment Organism
phosphatidylinositol 4-kinase type IIalpha
-
Homo sapiens
PI4K IIalpha
-
Homo sapiens