Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
phosphatidylinositol 4-kinase type IIalpha in complex with ATP, vapor diffusion method | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K165-K172 | when the entire membrane binding segment K165-K172 was mutated to alanines, the kinase is inactive | Homo sapiens |
N163A | the mutant shows about 10% of wild type activity | Homo sapiens |
N249A | the mutant shows slightly reduced wild type activity | Homo sapiens |
R275A | the mutant shows about 15% of wild type activity | Homo sapiens |
V339A | the mutant shows slightly reduced wild type activity | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol | Homo sapiens | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9BTU6 | - |
- |
Purification (Comment) | Organism |
---|---|
affinity, anion exchange column chromatography, and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol | - |
Homo sapiens | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphatidylinositol 4-kinase type IIalpha | - |
Homo sapiens |
PI4K IIalpha | - |
Homo sapiens |