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Literature summary for 2.7.1.67 extracted from

  • Westergren, T.; Ekblad, L.; Jergil, B.; Sommarin, M.
    Phosphatidylinositol 4-kinase associated with spinach plasma membranes. Isolation and characterization of two distinct forms (1999), Plant Physiol., 121, 507-516.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.67

Inhibitors

Inhibitors Comment Organism Structure
adenosine 35% inhibition at 1 mM, 20% inhibition at 4 mM and above, enzyme form QI and QII Spinacia oleracea
Wortmannin IC50 for QI is approximately 0.007 mM, enzyme form QII is inhibited 30% at 0.01 mM Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 phosphatidylinositol pH 7.5, enzyme form QII Spinacia oleracea
0.23
-
 phosphatidylinositol pH 7.5, enzyme form QI Spinacia oleracea
0.44
-
 ATP pH 7.5, enzyme form QII Spinacia oleracea
0.49
-
 ATP pH 7.5, enzyme form QI Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane firmly associated with plasma membrane Spinacia oleracea 16020
-
plasma membrane formyl associated with plasma membrane Spinacia oleracea 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ inhibits enzyme form QI and QII at millimolar concentrations Spinacia oleracea
Mg2+ Mg2+ is the preferred divalent cation Spinacia oleracea
Mn2+ Mn2+ can partially substitute for Mg2+ in the reaction catalyzed by the QII enzyme but not in that catalyzed by QI. Mn2+ acts synergistically, with suboptimal Mg2+ concentrations to activate not only the QII enzyme, but also to some extent QI Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Spinacia oleracea
120000
-
 x * 120000, SDS-PAGE Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, enzyme form QI and QII Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol
-
 Spinacia oleracea ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Spinacia oleracea
monomer x * 120000, SDS-PAGE Spinacia oleracea

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 pH 7.0: about 50% of maximal activity, pH 8.5: about 40% of maximal activity, enzyme form QII Spinacia oleracea
7 8 pH 7.0: about 70% of maximal activity, pH 8.0: about 45% of maximal activity Spinacia oleracea

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.007
-
 IC50 for QI is approximately 0.007 mM, enzyme form QII is inhibited 30% at 0.01 mM Spinacia oleracea Wortmannin