Cloned (Comment) | Organism |
---|---|
glutathione S-transferase PI4Kbeta fusion protein, expression in Escherichia coli | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | creation of a glutathione S-transferase PI4Kbeta fusion protein that is biologically active and phosphorylates phosphatidylinositol in ist 4-position with wortmannin sensitivity and kinetic parameters that are identical to those of purified wild-type enzyme | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | enzyme exhibits autophosphorylation that is enhanced by Mn2+ ions. Isolated C-terminal catalytic domain still displays autophosphorylation. Phosphorylation of endogenous or overexpressed PI4Kbeta is also observed in COS-7 cells | Bos taurus |
Purification (Comment) | Organism |
---|---|
glutathione S-transferase PI4Kbeta fusion protein | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol | - |
Bos taurus | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? |