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Literature summary for 2.7.1.66 extracted from
- Prokaryotic diacylglycerol kinase and undecaprenol kinase (2012), Annu. Rev. Biophys., 41, 81-101.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the enzyme is a possible anti-cariogenic drug target | Streptococcus mutans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | located in the plasma membrane with the active site facing the cytosol | Bacillus subtilis | 5886 | - |
| plasma membrane | located in the plasma membrane with the active site facing the cytosol | Escherichia coli | 5886 | - |
| plasma membrane | located in the plasma membrane with the active site facing the cytosol | Streptococcus mutans | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Bacillus subtilis |  |
| Mg2+ | required | Escherichia coli | |
| Mg2+ | required | Streptococcus mutans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
1 * 17000 | Streptococcus mutans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + undecaprenol | Bacillus subtilis | - |
ADP + undecaprenyl phosphate | - |
? | |
| ATP + undecaprenol | Escherichia coli | - |
ADP + undecaprenyl phosphate | - |
? | |
| ATP + undecaprenol | Streptococcus mutans | - |
ADP + undecaprenyl phosphate | - |
? | |
| additional information | Escherichia coli | the diacylglycerol kinase of Escherichia coli shows very low undecaprenol kinase, a true undecaprenol kinase is not found in Escherichia coli | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Streptococcus mutans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme | Streptococcus mutans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + undecaprenol | - |
Bacillus subtilis | ADP + undecaprenyl phosphate | - |
? | |
| ATP + undecaprenol | - |
Escherichia coli | ADP + undecaprenyl phosphate | - |
? | |
| ATP + undecaprenol | - |
Streptococcus mutans | ADP + undecaprenyl phosphate | - |
? | |
| additional information | the diacylglycerol kinase of Escherichia coli shows very low undecaprenol kinase, a true undecaprenol kinase is not found in Escherichia coli | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 17000 | Streptococcus mutans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDPK | - |
Bacillus subtilis |
| UDPK | - |
Escherichia coli |
| UDPK | - |
Streptococcus mutans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bacillus subtilis | |
| ATP | - |
Escherichia coli | |
| ATP | - |
Streptococcus mutans | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | prokaryotic diacylglycerol kinase and undecaprenol kinase are the lone members of the prokaryotic diacylglycerol kinase and undecaprenol kinase family of multispan membrane enzymes that are very small, lack relationships to any other family of proteins, including water soluble kinases, and that exhibit an unusual structure and active site architecture. The enzymes show complex structure, forming domain-swapped homotrimers in which each monomer subunit has three transmembrane segments. Members of this family are unrelated to the eukaryotic diacylglycerol kinases and their prokaryotic homologs, encoded by the dgkB gene | Streptococcus mutans |
| metabolism | UDPK activity does not participate in the de novo biosynthetic pathway to undecaprenyl phosphate which does not include free undecaprenol as an intermediate | Streptococcus mutans |
| physiological function | Gram-negative Escherichia coli does not contain high llevels of free undecaprenol, thus has no need for a undecaprenol kinase | Escherichia coli |
| physiological function | UDPK is essential for sporulation of Bacillus subtilis | Bacillus subtilis |
| physiological function | UDPK seems to play an analogous role in Gram-positive bacteria, where its importance is evident by the fact that UDPK is essential for biofilm formation by the oral pathogen Streptococcus mutans. Undecaprenol has an unusual function as covalent transmembrane carriers of hydrophilic molecules from the inner to the outer leaflet of the plasma membrane for use in biosynthesis of molecules located in the cell wall or periplasm. UDPK is an important enzyme in Streptococcus mutans required for growth at low pH, for biosynthesis of certain lantibiotics, and for biofilm formation.. It is also a virulence factor for the formation of smooth surface dental caries | Streptococcus mutans |
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