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Literature summary for 2.7.1.66 extracted from
- Biosynthesis of the peptidoglycan of bacterial cell walls. XIX. Isoprenoid alcohol phosphokinase (1970), J. Biol. Chem., 245, 3683-3690.
General Stability
| General Stability | Organism |
|---|---|
| relatively unstable in suspension in water | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | complete inhibition at 0.01 M | Staphylococcus aureus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
ATP | pH 8.2, 37°C, reaction with ficaprenol | Staphylococcus aureus | |
| 0.5 | - |
ficaprenol | pH 8.2, 37°C | Staphylococcus aureus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | exclusively found in the cell membrane fraction | Staphylococcus aureus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | no effect | Staphylococcus aureus | |
| Mg2+ | optimum concentration: 0.01 M | Staphylococcus aureus | |
| Mg2+ | divalent cation required, Mg2+ most effective, optimal concentration: 0.01 M | Staphylococcus aureus | |
| Mn2+ | can partially replace Mg2+ in activation | Staphylococcus aureus | |
| Mn2+ | one-fourth of the activity of Mg2+ at 0.001 M | Staphylococcus aureus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Staphylococcus aureus | product of the reaction is a substrate for the synthesis of lipid intermediates in peptidoglycan synthesis | ? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 1-butanol | highly stable | Staphylococcus aureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + ficaprenol | - |
Staphylococcus aureus | ADP + ficaprenyl phosphate | - |
? | |
| ATP + solanesol | no activity | Staphylococcus aureus | ADP + solanesyl phosphate | - |
? | |
| additional information | product of the reaction is a substrate for the synthesis of lipid intermediates in peptidoglycan synthesis | Staphylococcus aureus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
pH 8.2, aqueous suspension in Tris-HCl, presence of substrate, t1/2: 10 min | Staphylococcus aureus |
| 100 | - |
solution in 1-butanol, activation of nearly 50% after 20 min | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Staphylococcus aureus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 8.8 | pH 7.2: about 80% of maximal activity, pH 8.8: about 70% of maximal activity | Staphylococcus aureus |
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