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Literature summary for 2.7.1.63 extracted from

  • Mukai, T.; Kawai, S.; Matsukawa, H.; Matuo, Y.; Murata, K.
    Characterization and molecular cloning of a novel enzyme, inorganic polyphosphate/ATP-glucomannokinase, of Arthrobacter sp. strain KM (2003), Appl. Environ. Microbiol., 69, 3849-3857.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
Hexametaphosphate pH 7.0, 30°C Arthrobacter sp.
0.15
-
mannose pH 7.0, 30°C Arthrobacter sp.
0.2
-
ATP pH 7.0, 30°C Arthrobacter sp.
0.5
-
glucose pH 7.0, 30°C Arthrobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation required, 86% of the activity with Mg2+ for the polyphosphate-dependent activity and 30% of the activity with Mg2+ for ATP-dependent activity, 5 mM Arthrobacter sp.
Cu2+ divalent cation required, 11% of the activity with Mg2+ for the polyphosphate-dependent activity and no activation of ATP-dependent activity, 5 mM Arthrobacter sp.
Fe2+ divalent cation required, no activation of the polyphosphate-dependent activity and 15% of the activity with Mg2+ for ATP-dependent acticity, 5 mM Arthrobacter sp.
Mg2+ divalent cation required, maximal activition of polyphosphate-dependent glucokinase and ATP-dependent glucokinase activity, 5 mM Arthrobacter sp.
Mn2+ divalent cation required, 82% of the activity with Mg2+ for the polyphosphate-dependent activity and 88% of the activity with Mg2+ for ATP-dependent activity, 5 mM Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. KM
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
110
-
reaction with ATP and glucose Arthrobacter sp.
220
-
reaction with polyphosphate and glucose Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(phosphate)n + D-glucose
-
Arthrobacter sp. (phosphate)n-1 + D-glucose 6-phosphate
-
ir
(phosphate)n + D-glucose
-
Arthrobacter sp. KM (phosphate)n-1 + D-glucose 6-phosphate
-
ir
(phosphate)n + D-mannose about 40% of the activity with glucose Arthrobacter sp. (phosphate)n-1 + D-mannose 6-phosphate
-
?
(phosphate)n + D-mannose about 40% of the activity with glucose Arthrobacter sp. KM (phosphate)n-1 + D-mannose 6-phosphate
-
?
ATP + D-glucose
-
Arthrobacter sp. ADP + D-glucose 6-phosphate
-
?
ATP + D-glucose
-
Arthrobacter sp. KM ADP + D-glucose 6-phosphate
-
?
ATP + D-mannose about 30% of the activity with glucose Arthrobacter sp. ADP + D-mannose 6-phosphate
-
?
ATP + D-mannose about 30% of the activity with glucose Arthrobacter sp. KM ADP + D-mannose 6-phosphate
-
?
hexametaphosphate + D-glucose
-
Arthrobacter sp. pentametaphosphate + D-glucose 6-phosphate
-
?
additional information the enzyme also shows weak NAD kinase activity and fructokinase activity with polyphosphate or ATP Arthrobacter sp. ?
-
?
additional information the enzyme also shows weak NAD kinase activity and fructokinase activity with polyphosphate or ATP Arthrobacter sp. KM ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
polyphosphate-dependent and ATP-dependent mannokinase activity, polyphosphate and ATP-dependent glucokinase activity Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
5 min, 50% loss of activity Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
polyphosphate- and ATP-dependent mannokinase activity, polyphosphate and ATP-dependent glucokinase activity Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 pH 6.0: about 25% of maximal activity, pH 9.5: about 35% of maximal activity, polyphosphate-dependent and ATP-dependent mannokinase activity, polyphosphate and ATP-dependent glucokinase activity Arthrobacter sp.