Cloned (Comment) | Organism |
---|---|
- |
Arthrobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
Hexametaphosphate | pH 7.0, 30°C | Arthrobacter sp. | |
0.15 | - |
mannose | pH 7.0, 30°C | Arthrobacter sp. | |
0.2 | - |
ATP | pH 7.0, 30°C | Arthrobacter sp. | |
0.5 | - |
glucose | pH 7.0, 30°C | Arthrobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation required, 86% of the activity with Mg2+ for the polyphosphate-dependent activity and 30% of the activity with Mg2+ for ATP-dependent activity, 5 mM | Arthrobacter sp. | |
Cu2+ | divalent cation required, 11% of the activity with Mg2+ for the polyphosphate-dependent activity and no activation of ATP-dependent activity, 5 mM | Arthrobacter sp. | |
Fe2+ | divalent cation required, no activation of the polyphosphate-dependent activity and 15% of the activity with Mg2+ for ATP-dependent acticity, 5 mM | Arthrobacter sp. | |
Mg2+ | divalent cation required, maximal activition of polyphosphate-dependent glucokinase and ATP-dependent glucokinase activity, 5 mM | Arthrobacter sp. | |
Mn2+ | divalent cation required, 82% of the activity with Mg2+ for the polyphosphate-dependent activity and 88% of the activity with Mg2+ for ATP-dependent activity, 5 mM | Arthrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
- |
- |
Arthrobacter sp. KM | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Arthrobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
110 | - |
reaction with ATP and glucose | Arthrobacter sp. |
220 | - |
reaction with polyphosphate and glucose | Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(phosphate)n + D-glucose | - |
Arthrobacter sp. | (phosphate)n-1 + D-glucose 6-phosphate | - |
ir | |
(phosphate)n + D-glucose | - |
Arthrobacter sp. KM | (phosphate)n-1 + D-glucose 6-phosphate | - |
ir | |
(phosphate)n + D-mannose | about 40% of the activity with glucose | Arthrobacter sp. | (phosphate)n-1 + D-mannose 6-phosphate | - |
? | |
(phosphate)n + D-mannose | about 40% of the activity with glucose | Arthrobacter sp. KM | (phosphate)n-1 + D-mannose 6-phosphate | - |
? | |
ATP + D-glucose | - |
Arthrobacter sp. | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | - |
Arthrobacter sp. KM | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-mannose | about 30% of the activity with glucose | Arthrobacter sp. | ADP + D-mannose 6-phosphate | - |
? | |
ATP + D-mannose | about 30% of the activity with glucose | Arthrobacter sp. KM | ADP + D-mannose 6-phosphate | - |
? | |
hexametaphosphate + D-glucose | - |
Arthrobacter sp. | pentametaphosphate + D-glucose 6-phosphate | - |
? | |
additional information | the enzyme also shows weak NAD kinase activity and fructokinase activity with polyphosphate or ATP | Arthrobacter sp. | ? | - |
? | |
additional information | the enzyme also shows weak NAD kinase activity and fructokinase activity with polyphosphate or ATP | Arthrobacter sp. KM | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
polyphosphate-dependent and ATP-dependent mannokinase activity, polyphosphate and ATP-dependent glucokinase activity | Arthrobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
5 min, 50% loss of activity | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
polyphosphate- and ATP-dependent mannokinase activity, polyphosphate and ATP-dependent glucokinase activity | Arthrobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | pH 6.0: about 25% of maximal activity, pH 9.5: about 35% of maximal activity, polyphosphate-dependent and ATP-dependent mannokinase activity, polyphosphate and ATP-dependent glucokinase activity | Arthrobacter sp. |