Any feedback?
Literature summary for 2.7.1.62 extracted from
- Phosphoramidate-hexose transphosphorylase (1966), Methods Enzymol., 9, 403-407.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| F- | 1.5 mM, at pH 8.7 8% inhibition, at pH 6.5 90% inhibition | Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.5 | - |
glucose | 37°C, pH 8.7 | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoramidate + D-glucose | Escherichia coli | phosphoramidate, i.e. PNH2, is delivered by KO3PNH3 | D-glucose 1-phosphate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
Crookes strain, ATCC 8739 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 22.7 | - |
37°C, pH 8.7 | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C or -15°C, 6 years stable | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoramidate + 2-deoxy-D-glucose | - |
Escherichia coli | 2-deoxy-D-glucose 1-phosphate + NH3 | - |
? | |
| phosphoramidate + 3-O-methylglucose | - |
Escherichia coli | 3-O-methylglucose 1-phosphate + NH3 | - |
? | |
| phosphoramidate + D-glucose | glucose is best substrate, phosphoramidate, i.e. PNH2, is delivered by KO3PNH3 | Escherichia coli | D-glucose 1-phosphate + NH3 | - |
? | |
| phosphoramidate + D-glucose | phosphoramidate, i.e. PNH2, is delivered by KO3PNH3 | Escherichia coli | D-glucose 1-phosphate + NH3 | - |
? | |
| phosphoramidate + fructose | - |
Escherichia coli | fructose 1-phosphate + NH3 | - |
? | |
| phosphoramidate + glucosamine | - |
Escherichia coli | glucosamine 1-phosphate + NH3 | - |
? | |
| phosphoramidate + mannose | - |
Escherichia coli | mannose 1-phosphate + NH3 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
pH 7.5, more than 30 min, no loss of activity | Escherichia coli |
| 100 | - |
10 min, about 60% loss of activity | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
assay at | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
