Any feedback?
Literature summary for 2.7.1.61 extracted from
- Acyl phosphate:hexose phosphotransferase (hexose phosphate:hexose phosphotransferase) (1966), Methods Enzymol., 9, 392-396.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| stable to repeated freezing and thawing | Klebsiella aerogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-mannose | inhibits phosphorylation of D-glucose, competitive to acetyl phosphate or D-mannose 6-phosphate | Klebsiella aerogenes |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
D-glucose | pH 7.5, 25°C | Klebsiella aerogenes | |
| 0.4 | - |
acetyl phosphate | carbamoyl phosphate, D-mannose 6-phosphate, pH 7.5, 25°C | Klebsiella aerogenes | |
| 2 | - |
D-ribose 5-phosphate | pH 7.5, 25°C | Klebsiella aerogenes | |
| 12 | - |
D-mannose | pH 7.5, 25°C | Klebsiella aerogenes | |
| 67 | - |
mannitol | pH 7.5, 25°C | Klebsiella aerogenes | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + acetyl phosphate | Klebsiella aerogenes | - |
D-glucose 6-phosphate + acetate | - |
? | |
| D-glucose + acetyl phosphate | Klebsiella aerogenes PRL-R3 | - |
D-glucose 6-phosphate + acetate | - |
? | |
| D-glucose + D-mannose 6-phosphate | Klebsiella aerogenes | - |
D-glucose 6-phosphate + D-mannose | - |
? | |
| D-glucose + D-mannose 6-phosphate | Klebsiella aerogenes PRL-R3 | - |
D-glucose 6-phosphate + D-mannose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
PRL-R3 | - |
| Klebsiella aerogenes PRL-R3 | - |
PRL-R3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Klebsiella aerogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.3 | - |
pH 7.5, 25°C | Klebsiella aerogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable | Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + acetyl phosphate | - |
Klebsiella aerogenes | D-fructose 1-phosphate + D-fructose 6-phosphate + acetate | D-fructose 1-phosphate is the predominant product | ? | |
| D-glucose + acetyl phosphate | - |
Klebsiella aerogenes | D-glucose 6-phosphate + acetate | - |
? | |
| D-glucose + acetyl phosphate | - |
Klebsiella aerogenes PRL-R3 | D-glucose 6-phosphate + acetate | - |
? | |
| D-glucose + carbamoyl phosphate | - |
Klebsiella aerogenes | D-glucose 6-phosphate + carbamate | - |
? | |
| D-glucose + carbamoyl phosphate | - |
Klebsiella aerogenes PRL-R3 | D-glucose 6-phosphate + carbamate | - |
? | |
| D-glucose + D-fructose 1-phosphate | 15% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-fructose | - |
? | |
| D-glucose + D-fructose 6-phosphate | 25% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-fructose | - |
? | |
| D-glucose + D-glucose 1-phosphate | 5% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-glucose | - |
? | |
| D-glucose + D-mannose 6-phosphate | - |
Klebsiella aerogenes | D-glucose 6-phosphate + D-mannose | - |
? | |
| D-glucose + D-mannose 6-phosphate | 71% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-mannose | - |
r | |
| D-glucose + D-mannose 6-phosphate | - |
Klebsiella aerogenes PRL-R3 | D-glucose 6-phosphate + D-mannose | - |
? | |
| D-glucose + D-mannose 6-phosphate | 71% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes PRL-R3 | D-glucose 6-phosphate + D-mannose | - |
r | |
| D-glucose + D-ribose 5-phosphate | 18% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-ribose | - |
? | |
| D-glucose + D-sorbitol 6-phosphate | 2.5% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-sorbitol | - |
? | |
| D-mannitol + acetyl phosphate | - |
Klebsiella aerogenes | ? | - |
? | |
| D-mannose + acetyl phosphate | - |
Klebsiella aerogenes | D-mannose 6-phosphate + acetate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Klebsiella aerogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Klebsiella aerogenes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | pH 7.5: 50% of maximum activity, pH 9: maximum activity | Klebsiella aerogenes |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
