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Literature summary for 2.7.1.56 extracted from
- D-fructose-1-phosphate kinase (1975), Methods Enzymol., 42C, 63-66.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
D-Fructose 1-phosphate | pH 7.5, 25°C | Klebsiella aerogenes |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 40 mM, 3fold increase of activity | Klebsiella aerogenes | |
| Mg2+ | required for activity | Klebsiella aerogenes | |
| Mg2+ | maximal activity at a Mg2+/ATP ratio of at least 2 | Klebsiella aerogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
gel filtration | Klebsiella aerogenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
fructose 6-phosphate kinase-deficient mutant of a uracil auxotroph of strain PRL-R3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Klebsiella aerogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 47 | - |
- |
Klebsiella aerogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 100 mM (NH4)2SO4, pH 7.5, several months, no loss of activity | Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 1-phosphate | no activity with D-fructose 6-phosphate | Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
| ATP + D-fructose 1-phosphate | no activity with D-glucose 6-phosphate, D-glucose 1-phosphate | Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
| ATP + D-fructose 1-phosphate | specific for D-fructose 1-phosphate, no activity with L-fructose 1-phosphate, D-fructose, D-mannose 6-phosphate | Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
| ATP + D-fructose 1-phosphate | no activity with sorbose 1-phosphate | Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Klebsiella aerogenes |
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Release 2023.1 (January 2023)
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