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Literature summary for 2.7.1.55 extracted from

  • Gibbins, L.N.; Simpson, F.J.
    The purification and properties of D-allose-6-kinase from Aerobacter aerogenes (1963), Can. J. Biochem., 9, 769-779.
No PubMed abstract available

General Stability

General Stability Organism
GSH stabilizes Klebsiella aerogenes

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10 mM: 50% inhibition Klebsiella aerogenes
Hg2+ strong Klebsiella aerogenes
p-chloromercuribenzoate strong, addition of GSH reduces inhibition to 13% Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.98
-
D-allose 25°C, pH 6.5 Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 37% as effective as Mg2+ Klebsiella aerogenes
Mg2+ requirement Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-allose Klebsiella aerogenes first step of Embden-Meyerhof-Parnas pathway ADP + D-allose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.93
-
25°C, pH 6.5 Klebsiella aerogenes

Storage Stability

Storage Stability Organism
0°C, 0.01 M glycerol, 0.002 M KH2PO4, 0.001 M EDTA, 16 h, 70-80% remaining activity Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + allitol 2.8% of activity with D-allose Klebsiella aerogenes ADP + allitol 6-phosphate
-
?
ATP + D-allose
-
Klebsiella aerogenes ADP + D-allose 6-phosphate
-
?
ATP + D-allose first step of Embden-Meyerhof-Parnas pathway Klebsiella aerogenes ADP + D-allose 6-phosphate
-
?
ATP + D-galactose 3.5% of activity with D-allose Klebsiella aerogenes ADP + D-galactose 6-phosphate
-
?
ATP + D-glucose 20% of activity with D-allose Klebsiella aerogenes ADP + D-glucose 6-phosphate
-
?
ATP + D-ribose 6.3% of activity with D-allose Klebsiella aerogenes ADP + D-ribose 5-phosphate
-
?
additional information CTP: 50% of activity with ATP, ITP: 43% of activity with ATP Klebsiella aerogenes ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Klebsiella aerogenes

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 40% of maximal activity at pH 5, 50% of maximal activity at pH 7.5 Klebsiella aerogenes

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 0°C, 10 min, 10% remaining activity at pH 5, 80% remaining activity at pH 8 Klebsiella aerogenes