General Stability | Organism |
---|---|
GSH stabilizes | Klebsiella aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM: 50% inhibition | Klebsiella aerogenes | |
Hg2+ | strong | Klebsiella aerogenes | |
p-chloromercuribenzoate | strong, addition of GSH reduces inhibition to 13% | Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.98 | - |
D-allose | 25°C, pH 6.5 | Klebsiella aerogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 37% as effective as Mg2+ | Klebsiella aerogenes | |
Mg2+ | requirement | Klebsiella aerogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-allose | Klebsiella aerogenes | first step of Embden-Meyerhof-Parnas pathway | ADP + D-allose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.93 | - |
25°C, pH 6.5 | Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
0°C, 0.01 M glycerol, 0.002 M KH2PO4, 0.001 M EDTA, 16 h, 70-80% remaining activity | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + allitol | 2.8% of activity with D-allose | Klebsiella aerogenes | ADP + allitol 6-phosphate | - |
? | |
ATP + D-allose | - |
Klebsiella aerogenes | ADP + D-allose 6-phosphate | - |
? | |
ATP + D-allose | first step of Embden-Meyerhof-Parnas pathway | Klebsiella aerogenes | ADP + D-allose 6-phosphate | - |
? | |
ATP + D-galactose | 3.5% of activity with D-allose | Klebsiella aerogenes | ADP + D-galactose 6-phosphate | - |
? | |
ATP + D-glucose | 20% of activity with D-allose | Klebsiella aerogenes | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-ribose | 6.3% of activity with D-allose | Klebsiella aerogenes | ADP + D-ribose 5-phosphate | - |
? | |
additional information | CTP: 50% of activity with ATP, ITP: 43% of activity with ATP | Klebsiella aerogenes | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Klebsiella aerogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | 40% of maximal activity at pH 5, 50% of maximal activity at pH 7.5 | Klebsiella aerogenes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | 0°C, 10 min, 10% remaining activity at pH 5, 80% remaining activity at pH 8 | Klebsiella aerogenes |