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Literature summary for 2.7.1.52 extracted from

  • Quirk, S.
    Crystallization and preliminary X-ray characterization of the Vitis vinifera fucokinase:GDP-fucose pyrophosphorylase (2012), Acta Crystallogr. Sect. F, 68, 1109-1112.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.52

Cloned(Commentary)

Cloned (Comment) Organism
expression as fusion protein with maltose-binding protein in Escherichia coli strain BL21 (DE3) Vitis vinifera

Crystallization (Commentary)

Crystallization (Comment) Organism
purified apo-enzyme, mixing of 10 mg/ml protein in 10 mM Tris-HCl pH 7.5 with 9% w/v PEG 6K, 5% v/v ethylene glycol, 5% v/v glycerol, 25 mM HEPES pH 7.5, 2% tert-butanol, 1 mM MgCl2, 1mM MnCl2, 24°C, 20-30 days, X-ray diffraction structure determination and analysis at 2.6 A resolution Vitis vinifera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 ATP pH and temperature not specified in the publication Vitis vinifera

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Vitis vinifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
118800
-
 gel filtration Vitis vinifera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-fucose Vitis vinifera
-
 ADP + beta-L-fucose 1-phosphate
-
 ?
additional information Vitis vinifera fucokinase:GDP-fucose pyrophosphorylase is a dual activity enzyme metabolizing fucose and nucleotide-sugar ?
-
 ?

Organism

Organism UniProt Comment Textmining
Vitis vinifera
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant maltose-binding protein fusion protein from Escherichia coli strain BL21(DE3) by amylose affinity chromatography, cleavage of the tag by Genease I protease, followed by guanosine affinity chromatography and ultrafiltration Vitis vinifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-fucose
-
 Vitis vinifera ADP + beta-L-fucose 1-phosphate
-
 ?
additional information fucokinase:GDP-fucose pyrophosphorylase is a dual activity enzyme metabolizing fucose and nucleotide-sugar Vitis vinifera ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 118800 Vitis vinifera

Synonyms

Synonyms Comment Organism
FKP
-
 Vitis vinifera
fucokinase:GDP-fucose pyrophosphorylase
-
 Vitis vinifera
L-fucokinase:GDP-fucose pyrophosphorylase
-
 Vitis vinifera

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
 ATP pH and temperature not specified in the publication Vitis vinifera

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Vitis vinifera