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Literature summary for 2.7.1.52 extracted from

  • Kotake, T.; Hojo, S.; Tajima, N.; Matsuoka, K.; Koyama, T.; Tsumuraya, Y.
    A bifunctional enzyme with L-fucokinase and GDP-L-fucose pyrophosphorylase activities salvages free L-fucose in Arabidopsis (2008), J. Biol. Chem., 283, 8125-8135.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli with chaperones GroEL, GroES Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
G133A mutation abolishes GDP-L-fucose diphosphorylase activity, hardly affects fucokinase activity Arabidopsis thaliana
G830A mutation reduces fucokinase activity by more than 90%, while preserving the GDP-L-fucose diphosphorylase activity Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
ATP pH 7.5, 35°C Arabidopsis thaliana
1
-
L-fucose pH 7.5, 35°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ or Mn2+, absolutely required for fucokinase activity Arabidopsis thaliana
Mn2+ or Mg2+, absolutely required for fucokinase activity Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
116350
-
x * 116350, calculated, x * 120000, SDS-PAGE Arabidopsis thaliana
120000
-
x * 116350, calculated, x * 120000, SDS-PAGE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
bifunctional enzyme, L-fucokinase and GDP-L-fucose diphosphorylase activities
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information peptide sequence does not contain a secretory signal or transmembrane domain Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
recombinant protein with His-tag and fused to thioredoxin, partial purification Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
additional information expression in all tissues examined Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
82.9
-
pH 7.5, 35°C Arabidopsis thaliana

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, 1 mM PMSF, 0.2 M NaCl, 50 mM Tris-HCl buffer, pH 7.5, stable for 1 month Arabidopsis thaliana
4°C, 1 day, loss of 50% of GDP-L-fucose diphosphorylase activity Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-fucose
-
Arabidopsis thaliana ADP + beta-L-fucose 1-phosphate
-
?
additional information enzyme generates GDP-L-fucose from L-fucose 1-phosphate and GTP. No substrates for fucokinase activity are: L-arabinose, glucose, galactose, mannose, xylose, L-rhamnose, galactosamine, glucosamine, N-acetylglucosamine Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
? x * 116350, calculated, x * 120000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
Fkgp
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
optimum for fucokinase activity Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
loss of 99% of activity Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.34
-
L-fucose pH 7.5, 35°C Arabidopsis thaliana
0.38
-
ATP pH 7.5, 35°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
optimum for fucokinase activity Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana calculated
-
5.9