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Literature summary for 2.7.1.52 extracted from

  • Kilker, R.D.; Shuey, D.K.; Serif, G.S.
    Isolation and properties of porcine thyroid fucokinase (1979), Biochim. Biophys. Acta, 570, 271-283.
    View publication on PubMed
Search for more literature for 2.7.1.52

Activating Compound

Activating Compound Comment Organism Structure
GDP-alpha-D-mannose stimulation Sus scrofa

General Stability

General Stability Organism
unstable in the presence of KCl Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
ADP 4.9 mM, 96% inhibition, competitive with ATP Sus scrofa
AMP 5.0 mM, 14% inhibition in presence of ATP Sus scrofa
CDP 4.9 mM, 16% inhibition in presence of ATP Sus scrofa
CTP 4.9 mM, 30% inhibition in presence of ATP Sus scrofa
GDP-L-fucose competitive with L-fucose Sus scrofa
GTP 0.1 mM, 59% inhibition in presence of ATP Sus scrofa
TTP 4.8 mM, 45% inhibition in presence of ATP Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 L-fucose pH 7.4, 37°C Sus scrofa
1
-
 ATP pH 7.4, 37°C Sus scrofa
1
-
 ATP ATP in form of MgATP2- Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 4 * 78000, SDS-PAGE Sus scrofa
309200
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 6-deoxy-L-galactose Sus scrofa i.e. L-fucose, serves to utilize free L-fucose for glycoprotein synthesis ADP + 6-deoxy-L-galactose 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
thyroid gland
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.6
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, in the presence of 2-mercaptoethanol and glycerol, quite stable Sus scrofa
4°C, unstable in saline solution Sus scrofa
frozen, unstable in saline solution Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 6-deoxy-L-galactose highly specific for ATP Sus scrofa ADP + 6-deoxy-L-galactose 1-phosphate
-
 ?
ATP + 6-deoxy-L-galactose highly specific for L-fucose Sus scrofa ADP + 6-deoxy-L-galactose 1-phosphate
-
 ?
ATP + 6-deoxy-L-galactose i.e. L-fucose, serves to utilize free L-fucose for glycoprotein synthesis Sus scrofa ADP + 6-deoxy-L-galactose 1-phosphate
-
 ?
ATP + L-fucose
-
 Sus scrofa ADP + beta-L-fucose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 78000, SDS-PAGE Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Sus scrofa
6.6
-
-
 Sus scrofa