Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL | Pyrococcus horikoshii OT3 |
Crystallization (Comment) | Organism |
---|---|
precipitant: sodium citrate, pH 9.3, 293 K | Pyrococcus horikoshii OT3 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | amino-acid residues in the magnesium ion-binding site are conserved | Pyrococcus horikoshii OT3 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28950 | - |
3 * 28950 Da, crystal structure, calculated | Pyrococcus horikoshii OT3 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii OT3 | O58877 | - |
- |
Purification (Comment) | Organism |
---|---|
heat treatment (363 K, 13 min), centrifugation, ion exchange chromatography, hydroxyapatite chromatography, gel filtration, | Pyrococcus horikoshii OT3 |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 28950 Da, crystal structure, calculated | Pyrococcus horikoshii OT3 |
Synonyms | Comment | Organism |
---|---|---|
4-methyl-5-beta-hydroxyethylthiazole kinase | - |
Pyrococcus horikoshii OT3 |