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Literature summary for 2.7.1.50 extracted from

  • Kawasaki, Y.
    Copurification of hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase of Saccharomyces cerevisiae: characterization of hydroxyethylthiazole kinase as a bifunctional enzyme in the thiamine biosynthetic pathway (1993), J. Bacteriol., 175, 5153-5158.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
2-Aminohydroxyethylthiazole
-
Saccharomyces cerevisiae
PCMB 0.01 mM, 98.5% inhibition, inhibition prevented by addition of 2-mercaptoethanol Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0057
-
5-(2-hydroxyethyl)-4-methylthiazole pH 7.5, 37°C Saccharomyces cerevisiae
0.79
-
ATP pH 7.5, 37°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 10 mM, stimulates as effectively as Mg2+ Saccharomyces cerevisiae
Mg2+ 10 mM, stimulates as effectively as Co2+ Saccharomyces cerevisiae
Mn2+ stimulates, 42.7% as effectively as Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
8 * 60000, bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity, SDS-PAGE Saccharomyces cerevisiae
470000
-
bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity, gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-methyl-5-(2-hydroxyethyl)thiazole Saccharomyces cerevisiae the bifunctioinal enzyme hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase catalyzes two sequential steps in the synthesis of thiamin monophosphate from hydroxyethylthiazole ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
mutant resistant to 2-amino-4-methyl-5-beta-hydroxyethylthiazole, an antimetabolite of 4-methyl-5-beta-hydroxyethylthiazole, deficient in activity of both EC 2.5.1.3 and EC 2.7.1.50
-
Saccharomyces cerevisiae
-
bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity
-

Purification (Commentary)

Purification (Comment) Organism
bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.53
-
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-80°C, about 50% loss of activity after 1 month Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + 4-methyl-5-(2-hydroxyethyl)thiazole 3.4% of the activity with ATP Saccharomyces cerevisiae AMP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
?
ATP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
Saccharomyces cerevisiae ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
?
ATP + 4-methyl-5-(2-hydroxyethyl)thiazole the bifunctioinal enzyme hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase catalyzes two sequential steps in the synthesis of thiamin monophosphate from hydroxyethylthiazole Saccharomyces cerevisiae ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
?
ATP + 5-(2-hydroxyethyl)-4-methylthiazole
-
Saccharomyces cerevisiae ADP + 4-methyl-5-(2-phosphoethyl)-thiazole
-
?
CTP + 4-methyl-5-(2-hydroxyethyl)thiazole 13.7% of the activity with ATP Saccharomyces cerevisiae CDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
?
dATP + 4-methyl-5-(2-hydroxyethyl)thiazole 24.8% of the activity with ATP Saccharomyces cerevisiae dADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
?
GTP + 4-methyl-5-(2-hydroxyethyl)thiazole 3.2% of the activity with ATP Saccharomyces cerevisiae GDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
?
UTP + 4-methyl-5-(2-hydroxyethyl)thiazole 75.1% of the activity with ATP Saccharomyces cerevisiae UDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
?

Subunits

Subunits Comment Organism
octamer 8 * 60000, bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
7.4 9.2 pH 7.4: about 80% of activity maximum, pH 9.2: about 25% of activity maximum Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0021
-
2-Aminohydroxyethylthiazole pH 7.5, 37°C Saccharomyces cerevisiae