Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM105 | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop and sitting drop vapour diffusion methods using 17% (w/v) PEG 8000 and 120 mM LiCl | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
gel filtration | Escherichia coli |
54110 | - |
calculated from amino acid sequence | Escherichia coli |
Organic Solvent | Comment | Organism |
---|---|---|
2-mercaptoethanol | incubation over more than 5 h in fresh 20 mM 2-mercaptoethanol does not change the activity | Escherichia coli |
dithiothreitol | incubation over more than 5 h in fresh 25 mM dithiothreitol does not change the activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P32171 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography and Superdex 200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-fructose | - |
Escherichia coli | ADP + L-fructose 1-phosphate | - |
? | |
ATP + L-rhamnulose | - |
Escherichia coli | ADP + L-rhamnulose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52000, gel filtration | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
L-rhamnulose kinase | - |
Escherichia coli |
RhuK | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |