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Literature summary for 2.7.1.49 extracted from
- Involvement of thiaminase II encoded by the THI20 gene in thiamin salvage of Saccharomyces cerevisiae (2008), FEMS Yeast Res., 8, 266-275.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine | Saccharomyces cerevisiae | - |
ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | Q08224 | trifunctional enzyme: EC 3.5.99.2/EC 2.7.1.49/EC 2.7.4.7 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine | - |
Saccharomyces cerevisiae | ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine | - |
? | |
| additional information | Thi20p is involved in thiamine synthesis from pyrithiamin and oxythiamin | Saccharomyces cerevisiae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Thi20p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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