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Literature summary for 2.7.1.49 extracted from

  • Rapala-Kozik, M.; Olczak, M.; Ostrowska, K.; Starosta, A.; Kozik, A.
    Molecular characterization of thi3 gene involved in thiamine biosynthesis in Zea mays: cDNA sequence and enzymatic and structural properties of recombinant bifunctional protein with 4-amino-5-hydroxymethyl-2-methylpyrimidine (phosphate) kinase and thiamin (2007), Biochem. J., 408, 149-159.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Zea mays

Protein Variants

Protein Variants Comment Organism
M134K detrimental to HMP kinase activity Zea mays
M134K/T472D double mutant with 25% activity compared to the wild type enzyme Zea mays
Q373L 30% reduced HMP kinase activity Zea mays
Q98L detrimental to HMP kinase activity Zea mays
Q98L/Q373L double mutant with 10% activity compared to the wild type enzyme Zea mays
S444A without any effect on activity Zea mays
T472D 50% reduced HMP kinase activity Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47500
-
 2 * 47500, gel filtration Zea mays
57800
-
 calculated from amino acid sequence Zea mays
95000
-
 gel filtration Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Superdex 200 HR 10/50 gel filtration Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine
-
 Zea mays ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 47500, gel filtration Zea mays

Synonyms

Synonyms Comment Organism
4-amino-5-hydroxymethyl-2-methylpyrimidine (phosphate) kinase
-
 Zea mays
HMP kinase
-
 Zea mays
THI3 the enzyme shows two essentially different enzymatic activities of 4-amino-5-hydroxymethyl-2-methylpyrimidne (phosphate) kinase and thiamine monophosphate synthase Zea mays

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Zea mays

pI Value

Organism Comment pI Value Maximum pI Value
Zea mays calculated from amino acid sequence
-
 8.51