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Literature summary for 2.7.1.48 extracted from

  • Qian, Y.; Ding, Q.; Li, Y.; Zou, Z.; Yan, B.; Ou, L.
    Phosphorylation of uridine and cytidine by uridine-cytidine kinase (2014), J. Biotechnol., 188, 81-87.
    View publication on PubMed
Search for more literature for 2.7.1.48

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli
expressed in Escherichia coli BL21(DE3) cells Lactobacillus delbrueckii subsp. bulgaricus
gene uck, recombinant expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Escherichia coli
genes uck1 and uck2, recombinant expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Lactobacillus delbrueckii subsp. bulgaricus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli
Mg2+ required Lactobacillus delbrueckii subsp. bulgaricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
-
 Lactobacillus delbrueckii subsp. bulgaricus
23500
-
-
 Escherichia coli
24000
-
-
 Lactobacillus delbrueckii subsp. bulgaricus
24000
-
 x * 24000, SDS-PAGE Escherichia coli
24000
-
 x * 24000, SDS-PAGE Lactobacillus delbrueckii subsp. bulgaricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + CMP Escherichia coli
-
 ADP + CDP
-
 ?
ATP + CMP Lactobacillus delbrueckii subsp. bulgaricus
-
 ADP + CDP
-
 ?
ATP + CMP Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
-
 ADP + CDP
-
 ?
ATP + UMP Escherichia coli
-
 ADP + UDP
-
 ?
ATP + UMP Lactobacillus delbrueckii subsp. bulgaricus
-
 ADP + UDP
-
 ?
ATP + UMP Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
-
 ADP + UDP
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli
-
 gene uck
-
Lactobacillus delbrueckii subsp. bulgaricus
-
-
-
Lactobacillus delbrueckii subsp. bulgaricus
-
 genes uck1 and uck2
-
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
-
-
-
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
-
 genes uck1 and uck2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + CMP
-
 Escherichia coli ADP + CDP
-
 ?
ATP + CMP
-
 Lactobacillus delbrueckii subsp. bulgaricus ADP + CDP
-
 ?
ATP + CMP low activity with ATP as phosphate donor Escherichia coli ADP + CDP
-
 ?
ATP + CMP low activity with ATP as phosphate donor Lactobacillus delbrueckii subsp. bulgaricus ADP + CDP
-
 ?
ATP + CMP
-
 Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 ADP + CDP
-
 ?
ATP + CMP low activity with ATP as phosphate donor Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 ADP + CDP
-
 ?
ATP + UMP
-
 Escherichia coli ADP + UDP
-
 ?
ATP + UMP
-
 Lactobacillus delbrueckii subsp. bulgaricus ADP + UDP
-
 ?
ATP + UMP low activity with ATP as phosphate donor Escherichia coli ADP + UDP
-
 ?
ATP + UMP low activity with ATP as phosphate donor Lactobacillus delbrueckii subsp. bulgaricus ADP + UDP
-
 ?
ATP + UMP
-
 Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 ADP + UDP
-
 ?
ATP + UMP low activity with ATP as phosphate donor Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 ADP + UDP
-
 ?
CTP + CMP low activity with CTP as phosphate donor Escherichia coli CDP + CDP
-
 ?
CTP + CMP low activity with CTP as phosphate donor Lactobacillus delbrueckii subsp. bulgaricus CDP + CDP
-
 ?
CTP + UMP low activity with CTP as phosphate donor Escherichia coli CDP + UDP
-
 ?
CTP + UMP low activity with CTP as phosphate donor Lactobacillus delbrueckii subsp. bulgaricus CDP + UDP
-
 ?
GTP + CMP GTP is the best phosphate donor Escherichia coli GDP + CDP
-
 ?
GTP + CMP GTP is the best phosphate donor Lactobacillus delbrueckii subsp. bulgaricus GDP + CDP
-
 ?
GTP + cytidine
-
 Escherichia coli GDP + CMP
-
 ?
GTP + cytidine
-
 Lactobacillus delbrueckii subsp. bulgaricus GDP + CMP
-
 ?
GTP + UMP GTP is the best phosphate donor Escherichia coli CDP + UDP
-
 ?
GTP + UMP GTP is the best phosphate donor Lactobacillus delbrueckii subsp. bulgaricus CDP + UDP
-
 ?
GTP + uridine GTP is the best phosphate donor Escherichia coli GDP + UMP
-
 ?
GTP + uridine GTP is the best phosphate donor Lactobacillus delbrueckii subsp. bulgaricus GDP + UMP
-
 ?
additional information it is ineffective to use ATP, UTP or CTP as the phosphate donor Escherichia coli ?
-
 ?
additional information it is ineffective to use ATP, UTP or CTP as the phosphate donor Lactobacillus delbrueckii subsp. bulgaricus ?
-
 ?
additional information it is ineffective to use ATP, UTP or CTP as the phosphate donor Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 ?
-
 ?
UTP + CMP low activity with UTP as phosphate donor Escherichia coli UDP + CDP
-
 ?
UTP + CMP low activity with UTP as phosphate donor Lactobacillus delbrueckii subsp. bulgaricus UDP + CDP
-
 ?
UTP + UMP low activity with UTP as phosphate donor Escherichia coli UDP + UDP
-
 ?
UTP + UMP low activity with UTP as phosphate donor Lactobacillus delbrueckii subsp. bulgaricus UDP + UDP
-
 ?

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE Escherichia coli
? x * 24000, SDS-PAGE Lactobacillus delbrueckii subsp. bulgaricus
? x * 23500, recombinant enzyme, SDS-PAGE Escherichia coli
? x * 24000, recombinant enzyme Uck1, SDS-PAGE, x * 23000, recombinant enzyme Uck2, SDS-PAGE Lactobacillus delbrueckii subsp. bulgaricus

Synonyms

Synonyms Comment Organism
UCK
-
 Escherichia coli
UCK
-
 Lactobacillus delbrueckii subsp. bulgaricus
uridine-cytidine kinase
-
 Escherichia coli
uridine-cytidine kinase
-
 Lactobacillus delbrueckii subsp. bulgaricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Escherichia coli
37
-
-
 Lactobacillus delbrueckii subsp. bulgaricus
37
-
 assay at Escherichia coli
37
-
 assay at Lactobacillus delbrueckii subsp. bulgaricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 for phosphorylation of uridine Escherichia coli
7 8 for phosphorylation of uridine Lactobacillus delbrueckii subsp. bulgaricus
7 8 phosphorylation of uridine Escherichia coli
7 8 phosphorylation of uridine Lactobacillus delbrueckii subsp. bulgaricus
7.5 8 for phosphorylation of cytidine Escherichia coli
7.5 8 for phosphorylation of cytidine Lactobacillus delbrueckii subsp. bulgaricus
7.5 8 phosphorylation of cytidine Escherichia coli
7.5 8 phosphorylation of cytidine Lactobacillus delbrueckii subsp. bulgaricus

Cofactor

Cofactor Comment Organism Structure
ATP physiological phosphate donor Escherichia coli
ATP physiological phosphate donor Lactobacillus delbrueckii subsp. bulgaricus
CTP low activity Escherichia coli
CTP low activity Lactobacillus delbrueckii subsp. bulgaricus
GTP best in vitro phosphate donor Escherichia coli
GTP best in vitro phosphate donor Lactobacillus delbrueckii subsp. bulgaricus
UTP low activity Escherichia coli
UTP low activity Lactobacillus delbrueckii subsp. bulgaricus