Literature summary for 2.7.1.48 extracted from

Islam, M.R.; Kim, H.; Kang, S.W.; Kim, J.S.; Jeong, Y.M.; Hwang, H.J.; Lee, S.Y.; Woo, J.C.; Kim, S.G.
Functional characterization of a gene encoding a dual domain for uridine kinase and uracil phosphoribosyltransferase in Arabidopsis thaliana (2007), Plant Mol. Biol., 63, 465-477.

Search for more literature for 2.7.1.48

Cloned(Commentary)

Cloned (Comment)	Organism
-	Arabidopsis thaliana
expressed in Escherichia coli strain JM109	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	contrary to wild-type, enzyme T-DNA insertion mutant plants are not affected in growth when treated with 5-fluorouracil or 5-fluorouridine. Expression of UK/UPRT1 in upp and uppudk mutants of Escherichia coli supplied with 5-fluorouracil and 5-fluorouridine leads to growth inhibition. Identical results are obtained with 5-fluorouridine and 5-fluorouracil treatments when the uridine kinase and uracil phosphoribosyltransferase domains are separated by the introduction of translation initiation and stop codons prior to complementation into the upp-udk and upp mutants	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
53000	-	SDS-PAGE	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9FKS0	bifunctional uridine kinase/uracil phosphoribosyltransferase UK/UPRT1	-
Arabidopsis thaliana	Q9FKS0	uridine kinase-like protein; ecotype Col-0	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
flower	-	Arabidopsis thaliana	-
leaf	-	Arabidopsis thaliana	-
root	-	Arabidopsis thaliana	-
stem	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 5-fluorouridine	-	Arabidopsis thaliana	ADP + 5-fluoro-uridine monophosphate	-	?
ATP + uridine	-	Arabidopsis thaliana	ADP + UMP	-	?

Subunits

Subunits	Comment	Organism
?	x * 53000, calculated	Arabidopsis thaliana
More	N-terminal region of UK/UPRT1 contains an uridine kinase domain, and the C-terminal region consists of an uracil phosphoribosyltransferase domain	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
UK/UPRT1	double catalytic enzyme for uridine kinase (N-terminal domain) and uracil phosphoribosyltransferase (C-terminal domain)	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)