Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.48 extracted from

  • Anderson, E.P.
    Uridine-cytidine kinase from a murine neoplasm (1978), Methods Enzymol., 51, 314-321.
    View publication on PubMed
Search for more literature for 2.7.1.48

General Stability

General Stability Organism
some loss of activity after repeated freezing and thawing Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
5'-Amino-5-deoxyuridine
-
 Mus musculus
5'-Azido-5-deoxyuridine
-
 Mus musculus
5'-Deoxyuridine
-
 Mus musculus
5'-Iodo-5'-deoxyuridine
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 cytidine pH 7.5, 25°C Mus musculus
0.15
-
 uridine pH 7.5, 25°C Mus musculus
2.1
-
 ATP pH 7.5, 25°C, cosubstrate cytidine Mus musculus
3.6
-
 ATP pH 7.5, 25°C, cosubstrate uridine Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 13 mM, 66% of activity with Mg2+ Mus musculus
Fe2+ can partially replace Mg2+ Mus musculus
Fe2+ 13 mM, 65% of activity with Mg2+ Mus musculus
Mg2+ required for full activity Mus musculus
Mn2+ can partially replace Mg2+ Mus musculus
Mn2+ 13 mM, 80% of activity with Mg2+ Mus musculus
additional information not activated by Ca2+ and Cu2+ Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + uridine Mus musculus enzyme is part of the anabolic pathway by which the preformed pyrimidine nucleosides are salvaged for nucleic acid biosynthesis ADP + UMP
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose, ammonium sulfate, hydroxyapatite Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
mastocytoma cell P815 cell line Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.03
-
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-azacytidine
-
 Mus musculus ADP + 5-azacytidine 5'-monophosphate
-
 ?
ATP + 5-fluorouridine
-
 Mus musculus ADP + 5-fluorouridine 5'-monophosphate
-
 ?
ATP + 6-azacytidine
-
 Mus musculus ADP + 6-azacytidine 5'-monophosphate
-
 ?
ATP + 6-azauridine
-
 Mus musculus ADP + 6-azauridine 5'-monophosphate
-
 ?
ATP + cytidine 50% of activity with uridine Mus musculus ADP + CMP
-
 ?
ATP + uridine no activity with thymine riboside, deoxyribosides, cytosine arabinoside, adenosine, purine deoxyribosides, UMP, CMP and deoxyribosides Mus musculus ADP + UMP
-
 ?
ATP + uridine enzyme is part of the anabolic pathway by which the preformed pyrimidine nucleosides are salvaged for nucleic acid biosynthesis Mus musculus ADP + UMP
-
 ?
dATP + uridine 88% of activity with ATP Mus musculus dADP + UMP
-
 ?
dCTP + uridine 50% of activity with ATP Mus musculus dCDP + UMP
-
 ?
dGTP + uridine 21% of activity with ATP Mus musculus dGDP + UMP
-
 ?
dUTP + uridine 67% of activity with ATP Mus musculus dUDP + UMP
-
 ?
GTP + uridine 23% of the activity with ATP Mus musculus UMP + GDP
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Mus musculus