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Literature summary for 2.7.1.45 extracted from
- Gene expression and characterization of 2-keto-3-deoxygluconate kinase, a key enzyme in the modified Entner-Doudoroff pathway of the aerobic and acidophilic hyperthermophile Sulfolobus tokodaii (2007), Protein Expr. Purif., 54, 73-78.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain BL21(DE3) | Sulfurisphaera tokodaii |
| expression in Escherichia coli | Sulfurisphaera tokodaii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.027 | - |
2-dehydro-3-deoxy-D-gluconate | pH 8.5, 50°C | Sulfurisphaera tokodaii |  |
| 0.027 | - |
2-dehydro-3-deoxy-D-gluconate | in 50 mM Tris/HCl (pH 8.5) at 50°C | Sulfurisphaera tokodaii | |
| 0.057 | - |
ATP | pH 8.5, 50°C | Sulfurisphaera tokodaii | |
| 0.057 | - |
ATP | in 50 mM Tris/HCl (pH 8.5) at 50°C, with 2-dehydro-3-deoxy-D-gluconate as substrate | Sulfurisphaera tokodaii | |
| 0.11 | - |
ATP | in 50 mM Tris/HCl (pH 8.5) at 50°C, with 2-keto-D-gluconate as substrate | Sulfurisphaera tokodaii | |
| 1.3 | - |
2-keto-D-gluconate | pH 8.5, 50°C | Sulfurisphaera tokodaii | |
| 1.3 | - |
2-keto-D-gluconate | in 50 mM Tris/HCl (pH 8.5) at 50°C | Sulfurisphaera tokodaii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 10 mM, required for activity, 98% relative activity compared to Mg2+ | Sulfurisphaera tokodaii | |
| Co2+ | 98% of the activity with Mg2+ | Sulfurisphaera tokodaii | |
| Mg2+ | most effective | Sulfurisphaera tokodaii | |
| Mg2+ | 10 mM, required for activity, most effective cation | Sulfurisphaera tokodaii | |
| Mn2+ | 73% of the activity with Mg2+ | Sulfurisphaera tokodaii | |
| Mn2+ | 10 mM, required for activity, 73% relative activity compared to Mg2+ | Sulfurisphaera tokodaii | |
| additional information | Cu2+ and Ca2+ have no stimulatory effect on activity | Sulfurisphaera tokodaii | |
| additional information | divalent kation is required for activity such as Mg2+, Co2+, Ni2+, Zn2+, Mn2+ | Sulfurisphaera tokodaii | |
| Ni2+ | 92% of the activity with Mg2+ | Sulfurisphaera tokodaii | |
| Ni2+ | 10 mM, required for activity, 92% relative activity compared to Mg2+ | Sulfurisphaera tokodaii | |
| Zn2+ | 10 mM, required for activity, 75% relative activity compared to Mg2+ | Sulfurisphaera tokodaii | |
| Zn2+ | 75% of the activity with Mg2+ | Sulfurisphaera tokodaii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
4 * 30000, gel filtration | Sulfurisphaera tokodaii |
| 34480 | - |
calculated from amino acid sequence | Sulfurisphaera tokodaii |
| 34480 | - |
4 * 34480, calculated, 4 * 35000, SDS-PAGE | Sulfurisphaera tokodaii |
| 35000 | - |
SDS-PAGE | Sulfurisphaera tokodaii |
| 35000 | - |
4 * 34480, calculated, 4 * 35000, SDS-PAGE | Sulfurisphaera tokodaii |
| 120000 | - |
gel filtration | Sulfurisphaera tokodaii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfurisphaera tokodaii | - |
- |
- |
| Sulfurisphaera tokodaii | Q96XN9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Butyl Toyopearl column chromatography and Superdex 200 column gel filtration | Sulfurisphaera tokodaii |
| recombinant protein | Sulfurisphaera tokodaii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
development of spectrophotometric assay that uses three coupled reactions to measure activity. Assay displays a linear relationship between time and the increase in absorbance at 340 nm due to NADPH formation | Sulfurisphaera tokodaii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-dehydro-3-deoxy-D-gluconate | - |
Sulfurisphaera tokodaii | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
| ATP + 2-keto-D-gluconate | - |
Sulfurisphaera tokodaii | ADP + 6-phospho-2-keto-D-gluconate | - |
? | |
| GTP + 2-dehydro-3-deoxy-D-gluconate | 94% activity compared to the substrate combination with ATP | Sulfurisphaera tokodaii | GDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
| GTP + 2-dehydro-3-deoxy-D-gluconate | 94% of the activity with ATP | Sulfurisphaera tokodaii | GDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
| ITP + 2-dehydro-3-deoxy-D-gluconate | 66% activity compared to the substrate combination with ATP | Sulfurisphaera tokodaii | IDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
| ITP + 2-dehydro-3-deoxy-D-gluconate | 66% of the activity with ATP | Sulfurisphaera tokodaii | IDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
| additional information | does not accept ADP or AMP as phosphoryl donors | Sulfurisphaera tokodaii | ? | - |
? | |
| additional information | no substrate: UTP, CTP, ADP, GDP, IDP, UDP, CDP, and AMP, D-glucose, D-fructose, and 2-keto-3-deoxyoctonate | Sulfurisphaera tokodaii | ? | - |
? | |
| TTP + 2-dehydro-3-deoxy-D-gluconate | 43% activity compared to the substrate combination with ATP | Sulfurisphaera tokodaii | TDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
| TTP + 2-dehydro-3-deoxy-D-gluconate | 43% of the activity with ATP | Sulfurisphaera tokodaii | TDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 30000, gel filtration | Sulfurisphaera tokodaii |
| tetramer | 4 * 34480, calculated, 4 * 35000, SDS-PAGE | Sulfurisphaera tokodaii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2-keto-3-deoxygluconate kinase | - |
Sulfurisphaera tokodaii |
| KDGK | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Sulfurisphaera tokodaii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
65% of maximum activity | Sulfurisphaera tokodaii |
| 90 | - |
25% of maximum activity | Sulfurisphaera tokodaii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
the activity at 50°C is about 65% of the maximum | Sulfurisphaera tokodaii |
| 80 | - |
1 h, no loss of activity | Sulfurisphaera tokodaii |
| 80 | - |
the purified recombinant enzyme is extremely thermostable and retains full activity after heating at 80°C for 1 h | Sulfurisphaera tokodaii |
| 85 | - |
1 h, 50% residual activity | Sulfurisphaera tokodaii |
| 90 | - |
the activity at 90°C is only about 25% of that at 80°C | Sulfurisphaera tokodaii |
| 90 | - |
10 min, 10% residual activity | Sulfurisphaera tokodaii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.26 | - |
2-dehydro-3-deoxy-D-gluconate | pH 8.5, 50°C | Sulfurisphaera tokodaii | |
| 8.26 | - |
2-dehydro-3-deoxy-D-gluconate | in 50 mM Tris/HCl (pH 8.5) at 50°C | Sulfurisphaera tokodaii | |
| 26.3 | - |
2-keto-D-gluconate | pH 8.5, 50°C | Sulfurisphaera tokodaii | |
| 26.3 | - |
2-keto-D-gluconate | in 50 mM Tris/HCl (pH 8.5) at 50°C | Sulfurisphaera tokodaii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Sulfurisphaera tokodaii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | half of the maximum activity is obtained at pHs 6.5 and 9.5 | Sulfurisphaera tokodaii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
no activity | Sulfurisphaera tokodaii |
| 10 | - |
no activity | Sulfurisphaera tokodaii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Sulfurisphaera tokodaii | |
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