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Literature summary for 2.7.1.40 extracted from
- Consequences of phosphoenolpyruvate:sugar phosphotranferase system and pyruvate kinase isozymes inactivation in central carbon metabolism flux distribution in Escherichia coli (2012), Microb. Cell Fact., 11, 127.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of W3110 derivative strains VH33, VH34 and VH35, that lack the main phosphoenolpyruvate consumers phosphoenolpyruvate:sugar phosphotransferase system and pyruvate kinase isozymes PykA and PykF causing modifications on cell physiology, carbon flux distribution and aromatics production capacity, overview. The phosphoenolpyruvate:sugar phosphotransferase-deficient strain shows lower specific rates for growth, glucose consumption and acetate production as well as a higher biomass yield from glucose. The effects are even more pronounced by the additional inactivation of PykA or PykF. The wild-type and mutant strains are modified to overproduce L-phenylalanine. In resting cells experiments, compared to reference strain, a 10, 4 and 7fold higher aromatics yields from glucose are observed as consequence of PTS, PTS PykA and PTS PykF inactivation, overview | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate | Escherichia coli | - |
ADP + phosphoenolpyruvate | - |
? | |
| ATP + pyruvate | Escherichia coli K-12 W3110 | - |
ADP + phosphoenolpyruvate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
isozymes PykA and PykF encoded by genes pykA and pykF | - |
| Escherichia coli K-12 W3110 | - |
isozymes PykA and PykF encoded by genes pykA and pykF | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate | - |
Escherichia coli | ADP + phosphoenolpyruvate | - |
? | |
| ATP + pyruvate | - |
Escherichia coli K-12 W3110 | ADP + phosphoenolpyruvate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PYK | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | phosphoenolpyruvate enters the aromatic amino acids biosynthesis, metabolic flux distribution and Pyk activity in wild-type strain W3110 and in modified strains VH33, VH34 and VH35, overview | Escherichia coli |
| physiological function | phosphoenolpyruvate is a key central metabolism intermediate that participates in glucose transport, as precursor in several biosynthetic pathways and it is involved in allosteric regulation of glycolytic enzymes | Escherichia coli |
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