Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 2,6-bisphosphate | wild-type, S0.5 value 0.000082 mM | Leishmania mexicana |
Crystallization (Comment) | Organism |
---|---|
crystals grown with ammonium sulfate as precipitant adopt an active-like conformation, with sulfate ions at the active and effector sites. Crystal soaking in sulfate-free buffers induces major conformational changes in the tetramer. The unwinding of the Aalpha6' helix and the inward hinge movement of the B domain are coupled with a significant widening of the tetramer caused by lateral movement of the C-domains | Leishmania mexicana |
Protein Variants | Comment | Organism |
---|---|---|
D315N | 3.7% of wild-type activity. S0.5 value for fructose 2,6-bisphosphate 0.001 mM | Leishmania mexicana |
D315S | 1.4% of wild-type activity. S0.5 value for fructose 2,6-bisphosphate 0.00161 mM | Leishmania mexicana |
E451W | 72% of wild-type activity. S0.5 value for fructose 2,6-bisphosphate 0.000177 mM | Leishmania mexicana |
S314N | 69% of wild-type activity. S0.5 value for fructose 2,6-bisphosphate 0.000403 mM | Leishmania mexicana |
S314Q | 48% of wild-type activity. S0.5 value for fructose 2,6-bisphosphate 0.000122 mM | Leishmania mexicana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | S0.5 value of phosphoenolpyruvate is 1.06 mM for wild-type, 1.3 mM for mutant E451W | Leishmania mexicana | |
0.214 | - |
ADP | wild-type, pH 7.5 | Leishmania mexicana | |
0.214 | - |
ADP | mutant E451W, pH 7.5 | Leishmania mexicana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania mexicana | Q27686 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | - |
Leishmania mexicana | ATP + pyruvate | - |
? |