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Literature summary for 2.7.1.40 extracted from
- Purification and characterization of cytosolic pyruvate kinase from leaves of the castor oil plant (1996), Arch. Biochem. Biophys., 333, 298-307.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| citrate | - |
Ricinus communis |  |
| L-glutamate | - |
Ricinus communis | |
| oxalate | - |
Ricinus communis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
phosphoenolpyruvate | pH 7.5, 30°C | Ricinus communis | |
| 0.037 | - |
ADP | pH 7.5, 30°C | Ricinus communis | |
| 0.037 | - |
ADP | ADP in form of MgADP- | Ricinus communis | |
| 0.087 | - |
ADP | pH 6.5, 30°C | Ricinus communis | |
| 0.087 | - |
ADP | ADP in form of MgADP- | Ricinus communis | |
| 0.089 | - |
phosphoenolpyruvate | pH 6.5, 30°C | Ricinus communis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Ricinus communis | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | requirement | Ricinus communis | |
| Mg2+ | requirement | Ricinus communis | |
| additional information | absolute requirement for a bivalent and a monovalent cation with Mg2+ and K+ fulfilling this | Ricinus communis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
2 * 56000 + 2 * 57000, SDS-PAGE | Ricinus communis |
| 57000 | - |
2 * 56000 + 2 * 57000, SDS-PAGE | Ricinus communis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ricinus communis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Ricinus communis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 51 | - |
pH 6.5, 30°C | Ricinus communis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | - |
Ricinus communis | ATP + pyruvate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 56000 + 2 * 57000, SDS-PAGE | Ricinus communis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
5 min, 10% loss of activity | Ricinus communis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
broad | Ricinus communis |
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Release 2023.1 (January 2023)
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