Any feedback?
Literature summary for 2.7.1.40 extracted from
- Pyruvate kinase isozymes from the green alga, Selenastrum minutum. II. Kinetic and regulatory properties (1989), Arch. Biochem. Biophys., 269, 228-238.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| asparagine | activation | Monoraphidium minutum |  |
| asparagine | kinetics | Monoraphidium minutum | |
| D-glucose 1-phosphate | slight activation, not isozyme PKp | Monoraphidium minutum | |
| D-glucose 6-phosphate | activation | Monoraphidium minutum | |
| D-glucose 6-phosphate | not: isozyme PKp | Monoraphidium minutum | |
| dihydroxyacetone phosphate | activation | Monoraphidium minutum | |
| dihydroxyacetone phosphate | kinetics | Monoraphidium minutum | |
| isoleucine | slight activation, kinetics | Monoraphidium minutum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-diphosphoglycerate | - |
Monoraphidium minutum | |
| 2-phosphoglycerate | only isozyme PKp, not PKc | Monoraphidium minutum | |
| 3-phosphoglycerate | - |
Monoraphidium minutum | |
| aspartate | only isozyme PKp, not PKc | Monoraphidium minutum | |
| ATP | MgATP2- | Monoraphidium minutum | |
| citrate | - |
Monoraphidium minutum | |
| D-ribose 5-phosphate | only isozyme PKp, not PKc | Monoraphidium minutum | |
| D-ribulose 1,5-bisphosphate | isozyme PKp, not PKc | Monoraphidium minutum | |
| fructose 1,6-diphosphate | - |
Monoraphidium minutum | |
| glucose 6-phosphate | only isozyme PKp, not PKc | Monoraphidium minutum | |
| isocitrate | only isozyme PKp, not PKc | Monoraphidium minutum | |
| malate | only isozyme PKp, not PKc | Monoraphidium minutum | |
| MgATP2- | - |
Monoraphidium minutum | |
| oxalate | - |
Monoraphidium minutum | |
| phosphate | - |
Monoraphidium minutum | |
| Phosphoglycolate | only isozyme PKp, not PKc | Monoraphidium minutum | |
| tyrosine | - |
Monoraphidium minutum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | 0.05 | ADP | PKc-isozyme | Monoraphidium minutum | |
| 0.049 | 0.1 | IDP | cosubstrate phosphoenolpyruvate | Monoraphidium minutum | |
| 0.049 | 0.1 | IDP | isozyme PKc | Monoraphidium minutum | |
| 0.18 | - |
phosphoenolpyruvate | isozyme PKp | Monoraphidium minutum | |
| 0.2 | 0.35 | ADP | PKp-isozyme | Monoraphidium minutum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | 2 isozymes: a cytosolic and a chloroplastid one | Monoraphidium minutum | 9507 | - |
| cytosol | 2 isozymes: a cytosolic and a plastid one | Monoraphidium minutum | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| divalent cation | requirement | Monoraphidium minutum | |
| divalent cation | requires both a divalent and a monovalent cation | Monoraphidium minutum | |
| K+ | - |
Monoraphidium minutum | |
| Mg2+ | - |
Monoraphidium minutum | |
| Mn2+ | requirement | Monoraphidium minutum | |
| Mn2+ | can partially replace Mg2+ | Monoraphidium minutum | |
| monovalent cation | requirement | Monoraphidium minutum | |
| monovalent cation | requires both a divalent and a monovalent cation | Monoraphidium minutum | |
| NH4+ | requirement | Monoraphidium minutum | |
| NH4+ | can replace K+ | Monoraphidium minutum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Monoraphidium minutum | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Monoraphidium minutum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | best nucleoside diphosphate substrate | Monoraphidium minutum | ATP + pyruvate | - |
? | |
| CDP + phosphoenolpyruvate | less effective than ADP | Monoraphidium minutum | CTP + pyruvate | - |
? | |
| CDP + phosphoenolpyruvate | reaction with PKp-isozyme at 30%, with PKc-isozyme at 12% the rate of ADP | Monoraphidium minutum | CTP + pyruvate | - |
? | |
| GDP + phosphoenolpyruvate | less effective than ADP | Monoraphidium minutum | GTP + pyruvate | - |
? | |
| GDP + phosphoenolpyruvate | reaction for PKc-isozyme at 71%, for PKp-isozyme at 39% the rate of ADP | Monoraphidium minutum | GTP + pyruvate | - |
? | |
| IDP + phosphoenolpyruvate | less effective than ADP | Monoraphidium minutum | ITP + pyruvate | - |
? | |
| IDP + phosphoenolpyruvate | reaction with PKp-isozyme at 20%, with PKc-isozyme at 89% the rate of ADP | Monoraphidium minutum | ITP + pyruvate | - |
? | |
| UDP + phosphoenolpyruvate | reaction at about 70% the rate of ADP, PKc-isozyme | Monoraphidium minutum | UTP + pyruvate | - |
? | |
| UDP + phosphoenolpyruvate | less effective than ADP | Monoraphidium minutum | UTP + pyruvate | - |
? | |
| UDP + phosphoenolpyruvate | reaction at about 31% the rate of ADP, PKp-isozyme | Monoraphidium minutum | UTP + pyruvate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Monoraphidium minutum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
isozyme PKp | Monoraphidium minutum |
| 7 | - |
isozyme PKc | Monoraphidium minutum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 8 | isozyme PKp, about half-maximal activity at pH 5.2 and pH 8 | Monoraphidium minutum |
| 5.9 | 8.2 | isozyme PKc, about half-maximal activity at pH 5.9 and pH 8.2 | Monoraphidium minutum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
