Any feedback?
Literature summary for 2.7.1.40 extracted from
- Purification and properties of mouse pyruvate kinases K and M and of a modified K subunit (1981), Biochemistry, 20, 1497-1506.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 1,6-diphosphate | activation | Mus musculus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic parameters | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Mus musculus |
| 58600 | - |
4 * 58600, M4-type isozyme, SDS-PAGE | Mus musculus |
| 59500 | - |
4 * 59500, K4-type isozyme, SDS-PAGE | Mus musculus |
| 220000 | - |
M4-type isozyme, glycerol density gradient sedimentation | Mus musculus |
| 225500 | - |
M4-type isozyme, PAGE | Mus musculus |
| 230000 | - |
K4-type isozyme, glycerol density gradient sedimentation, PAGE | Mus musculus |
| 238000 | - |
K4-type isozyme, gel filtration | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| K4- and M4-isozymes and a hybrid form | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | skeletal | Mus musculus | - |
| muscle | type M-isozyme | Mus musculus | - |
| spleen | K-type isozyme | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 380 | - |
M-type isozyme | Mus musculus |
| 397.5 | - |
K-type isozyme | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | - |
Mus musculus | ATP + pyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 59500, K4-type isozyme, SDS-PAGE | Mus musculus |
| tetramer | 4 * 58600, M4-type isozyme, SDS-PAGE | Mus musculus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Mus musculus | K4-type isozyme, isoelectric focusing | - |
6.2 |
| Mus musculus | M4-type isozyme, isoelectric focusing | 7.4 | 7.3 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
