Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-diphosphate | activation | Bos taurus | |
D-fructose 1,6-diphosphate | not | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
phosphoenolpyruvate | cosubstrate ADP | Bos taurus | |
0.18 | - |
phosphoenolpyruvate | L-type isozyme, 25°C, pH 7.0 | Bos taurus | |
0.2 | 0.35 | ADP | M-type isozyme, 25°C, pH 7.0 | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
divalent cation | requirement | Bos taurus | |
K+ | - |
Bos taurus | |
Mg2+ | activation | Bos taurus | |
monovalent cation | requirement | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
4 * 52000, L-type isozyme | Bos taurus |
215000 | - |
L-type isozyme | Bos taurus |
230000 | - |
M-type isozyme | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | Bos taurus | allosteric enzyme | ATP + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mammalian tissues contain at least 3 distinct and electrophoretically separable kinase isozymes: K-type predominant in fetal tissues, L-type in adult liver and kidney, M-type in brain, skeletal and cardiac muscle | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
233 | - |
25°C, pH 7.0, M-type isozyme from neck muscle | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | 4 phosphoenolpyruvate-binding sites/enzyme molecule | Bos taurus | ATP + pyruvate | - |
? | |
ADP + phosphoenolpyruvate | positive cooperativity for phosphoenolpyruvate | Bos taurus | ATP + pyruvate | - |
? | |
ADP + phosphoenolpyruvate | allosteric enzyme | Bos taurus | ATP + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the isozymes are thought to be homotetramers, but hybrid isozymes result in vivo if a cell synthesizes 2 or more subunits simultaneously and in vitro after denaturation/renaturation of isozymic mixtures | Bos taurus |
tetramer | 4 * 52000, L-type isozyme | Bos taurus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bos taurus | L-type isozyme | - |
5.1 |
Bos taurus | M-type isozyme | - |
8.9 |
Bos taurus | K-type isozyme | - |
9 |