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Literature summary for 2.7.1.40 extracted from

  • Cardenas, J.M.
    Pyruvate kinase from bovine muscle and liver (1982), Methods Enzymol., 90, 140-149.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 1,6-diphosphate activation Bos taurus
D-fructose 1,6-diphosphate not Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
phosphoenolpyruvate cosubstrate ADP Bos taurus
0.18
-
phosphoenolpyruvate L-type isozyme, 25°C, pH 7.0 Bos taurus
0.2 0.35 ADP M-type isozyme, 25°C, pH 7.0 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
divalent cation requirement Bos taurus
K+
-
Bos taurus
Mg2+ activation Bos taurus
monovalent cation requirement Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
4 * 52000, L-type isozyme Bos taurus
215000
-
L-type isozyme Bos taurus
230000
-
M-type isozyme Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + phosphoenolpyruvate Bos taurus allosteric enzyme ATP + pyruvate
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mammalian tissues contain at least 3 distinct and electrophoretically separable kinase isozymes: K-type predominant in fetal tissues, L-type in adult liver and kidney, M-type in brain, skeletal and cardiac muscle Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Bos taurus
233
-
25°C, pH 7.0, M-type isozyme from neck muscle Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate 4 phosphoenolpyruvate-binding sites/enzyme molecule Bos taurus ATP + pyruvate
-
?
ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Bos taurus ATP + pyruvate
-
?
ADP + phosphoenolpyruvate allosteric enzyme Bos taurus ATP + pyruvate
-
?

Subunits

Subunits Comment Organism
More the isozymes are thought to be homotetramers, but hybrid isozymes result in vivo if a cell synthesizes 2 or more subunits simultaneously and in vitro after denaturation/renaturation of isozymic mixtures Bos taurus
tetramer 4 * 52000, L-type isozyme Bos taurus

pI Value

Organism Comment pI Value Maximum pI Value
Bos taurus L-type isozyme
-
5.1
Bos taurus M-type isozyme
-
8.9
Bos taurus K-type isozyme
-
9