Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose | substrate inhibition, inhibitory above 1 mM | Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.156 | - |
D-fructose | pH 7, 37°C | Entamoeba histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimum concentration 10 mM | Entamoeba histolytica | |
Mn2+ | 83% of the activity with Mg2+ | Entamoeba histolytica | |
additional information | no activity with Ca2+ | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33600 | - |
- |
Entamoeba histolytica |
35000 | - |
- |
Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | C4M2I2 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26.3 | - |
pH 7, 37°C | Entamoeba histolytica |
Storage Stability | Organism |
---|---|
-20°C in 50% (v/v) glycerol, stable for at least 6 months | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | - |
Entamoeba histolytica | ADP + D-fructose 6-phosphate | - |
? | |
ATP + D-mannose | - |
Entamoeba histolytica | ADP + D-mannose 6-phosphate | - |
? | |
additional information | no substrates: glucose, galactose | Entamoeba histolytica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE, x * 33600, calculated | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
CL6EHI_054510 | - |
Entamoeba histolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Entamoeba histolytica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.3 | - |
D-fructose | pH 7, 37°C | Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Entamoeba histolytica |
Organism | Comment | Expression |
---|---|---|
Entamoeba histolytica | fructokinase messenger RNA is modestly but significantly upregulated in amoebae switched to fructose medium as well as in fructose-adapted Entamoeba histolytica | up |