Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
the crystal structures of ROK FK from Bacillus subtilis (a) apo and in the presence of (b) ADP and (c) ADP/D-fructose is shown. All structures show that YdhR is a homo-dimer with a monomer composed of two similar alpha/beta domains forming a large cleft between domains that bind ADP and D-fructose | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
G59A | point mutation of glycine 59 to alanine shows that this residue plays a critical role in specificity of YdhR to D-fructose. Point mutation nearly completely abolishes the fructokinase activity | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O05510 | - |
- |
Bacillus subtilis 168 | O05510 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | - |
Bacillus subtilis | ADP + D-fructose 6-phosphate | - |
? | |
ATP + D-fructose | - |
Bacillus subtilis 168 | ADP + D-fructose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | crystal structure | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
ROK FK | - |
Bacillus subtilis |
YdhR | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bacillus subtilis |